MBL Overexpression Lysate (Native)

Images

 
Western Blot: Mannan Binding Lectin Overexpression Lysate (Adult Normal) [NBL1-12927] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Mannan Binding Lectin.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MBL Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC210143 and based on accession number NM_000242. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens mannose-binding lectin (protein C) 2, soluble (opsonic defect) (MBL2), mRNA.
Gene
MBL2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MBL

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MBL Overexpression Lysate (Native)

  • COLEC1
  • COLEC1collectin-1
  • Collectin-1
  • HSMBPC
  • Mannan-binding protein
  • mannose-binding lectin (protein C) 2, soluble (opsonic defect)
  • mannose-binding lectin (protein C) 2, soluble
  • Mannose-binding lectin
  • mannose-binding protein C
  • MBL
  • MBL2
  • MBL2D
  • MBLmannan-binding lectin
  • MBP
  • MBP1
  • MBP1mannose-binding lectin 2, soluble (opsonic defect)
  • MBP-C
  • MGC116832
  • MGC116833

Background

This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes mannose and N-acetylglucosamine on many microorganisms, and is capable of activating the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases. [provided by RefSeq]. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for MBL Lysate (NBL1-12927) (0)

There are no publications for MBL Lysate (NBL1-12927).
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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol MBL2
Entrez