MBL Antibody (1E2) - BSA Free

Product Discontinued

MBL Antibody (1E2) - BSA Free Summary

• Immunogen: MBL purified from human donor plasma and adsorbed onto aluminum hydroxide.
• Epitope: The epitope is thought to be on the carbohydrate recognition domain and differs from that of Clone 01 and Clone 11.
• Specificity: Clone 1E2 is specific for MBL from human serum or plasma.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MBL2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Clone 1E2 reacts strongly with MBL. Strong reaction is seen in ELISA with MBL coated directly onto the microtiter well. In Western blotting Clone 1E2 reacts with human MBL in both its oligomerized state and as single protein chain of 26 kDa. Clone 1E2 can be used to measure MBL in plasma or serum but is not specific for oligomerized MBL when used in combination with itself or other MBL antibodies.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for MBL Antibody (1E2) - BSA Free

• COLEC1
• COLEC1collectin-1
• Collectin-1
• HSMBPC
• Mannan-binding protein
• mannose-binding lectin (protein C) 2, soluble (opsonic defect)
• mannose-binding lectin (protein C) 2, soluble
• Mannose-binding lectin
• mannose-binding protein C
• MBL
• MBL2
• MBL2D
• MBLmannan-binding lectin
• MBP
• MBP1
• MBP1mannose-binding lectin 2, soluble (opsonic defect)
• MBP-C
• MGC116832
• MGC116833

Background

Mannan-binding lectin (MBL), also called mannose-binding lectin or protein, belongs to the C-type family of collectins, showing calcium-dependent binding to certain sugars It consists of oligomers of triple-chain subunits and its binding and complement activating activities depend on its normal oligomerization. On binding to mannan-like microbial surface carbohydrates, MBL activates the complement system by means of its own lectin pathway, depending on the MBL-associated serine proteases (MASPs). Because of the presence of different structural and promoter alleles in the population, 12% or more of the population have low concentrations (<50ng/mL) of normally oligomerized, functional MBL in plasma or serum .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MBL2