MBD4 Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Raji human Burkitt's lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse MBD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5935) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MBD4 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MBD4 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human MBD4
  Met382-Ser580
  Accession # O95243
• Specificity: Detects human and mouse MBD4 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MBD4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MBD4 Antibody [Unconjugated]

• EC 3.2.2.-
• G/T mismatch glycosylase
• G/U mismatch glycosylase
• MBD4
• MED1G/5-fluorouracil mismatch glycosylase with biphasic kinetics
• methyl-CpG binding domain protein 4
• methyl-CpG-binding domain protein 43,N(4)-ethenocytosine glycosylase
• Methyl-CpG-binding endonuclease 1
• Methyl-CpG-binding protein MBD4
• Mismatch-specific DNA N-glycosylase
• putative methyl-CpG binding protein

Background

MBD4 (Methyl CpG-binding domain protein 4; also MED1) is a 65-68 kDa member of the methyl CpG binding protein family. It is expressed in non-germline cells, and serves at least two functions: one, it interacts with FADD to up- or down-regulate apoptosis, and two, it acts as a DNA N-glycosylase that excises improper chromosomal thymines that are created through the spontaneous deamination of methylcytosine to thymine. Human MDB4 is 580 amino acids (aa) in length. It contains one DNA-binding MBD domain (aa 76-148) and one endonuclease domain (aa 455-580). There are three isoform variants. One shows a deletion of aa 395-400, another shows an AlaPro substitution for aa 539-580, and a third shows a Gln substitution for aa 83-401. Over aa 382-580, human MBD4 shares 90% aa identity with mouse MBD4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MBD4 Antibody (AF5935)(1)

Publication using AF5935

• Bilardi, RA;Flensburg, C;Xu, Z;Derrick, EB;Kueh, A;Majewski, IJ; Mbd4 and MutS? protect cells from spontaneous deamination of 5-methylcytosine Nucleic acids research 2026-01-14 [PMID: 41556338] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: MBD4
• Uniprot:
  • O95243(Human)