MBD1 Antibody - BSA Free

Western Blot: MBD1 Antibody [NBP3-18674] - Figure 2. Western blot analysis using the antibody directed against MBD1 HeLa cells were transfected with an expression vector for TY1-tagged MBD1. The presence of the TY1-MBD1 in the cell lysates was demonstrated by western blot analysis with the antibody against MBD1 diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.

ELISA: MBD1 Antibody [NBP3-18674] - Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human MBD1 in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:54,000.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.6 mg/ml

MBD1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MBD1 Antibody - BSA Free (NBP3-18674) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Polyclonal antibody raised in rabbit against human MBD1 (Methyl-CpG-binding domain protein 1), using 3 KLH-conjugated synthetic peptides, two containing a sequence from the C-terminal part and one containing a sequence from the N-terminal part of the protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MBD1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide and 0.05% ProClin 300
• Concentration: 0.6 mg/ml
• Purity: Affinity purified

Alternate Names for MBD1 Antibody - BSA Free

• CXXC-type zinc finger protein 3
• methyl-CpG binding domain protein 1 isoform PCM1
• methyl-CpG binding domain protein 1
• methyl-CpG-binding domain protein 1
• Methyl-CpG-binding protein MBD1
• PCM1CXXC3RFT
• Protein containing methyl-CpG-binding domain 1
• the regulator of fibroblast growth factor 2 (FGF-2) transcription

Background

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MBD1
• Uniprot:
  • Q9UIS9(Human)