Max Antibody (103) - Azide and BSA Free

Western Blot: Max Antibody (103) [NBP2-90153] - Lane A: Jurkat Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:18 kDaObserved band size:20 kDa(We are unsure as to the identity of these extra bands.)

Flow Cytometry: Max Antibody (103) [NBP2-90153] - Analysis of Human MAX expression on Raji cells stained with purified anti-Human MAX, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Immunoprecipitation: Max Antibody (103) [NBP2-90153] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-MAX rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MAX rabbit monoclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size: 23 kDa

Immunocytochemistry/Immunofluorescence: Max Antibody (103) [NBP2-90153] - Staining of Human MAX in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90153 antibody (1:300) at 37? 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IP
• Clone: 103
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Max Antibody (103) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Human Max (Accession#: NP_002373; Met1-Ser160).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAX
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:25000-1:50000
  • Flow Cytometry 1:100-1:500
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunoprecipitation 0.2-1 uL/mg of lysate
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Max Antibody (103) - Azide and BSA Free

• BHLHD4
• bHLHd4MGC11225
• bHLHd5
• bHLHd6
• bHLHd7
• bHLHd8
• Class D basic helix-loop-helix protein 4
• helix-loop-helix zipper protein
• MAX protein
• Max
• MGC10775
• MGC18164
• MGC34679
• MGC36767
• MYC associated factor X
• Myc-associated factor X
• orf1
• protein max

Background

MAX is a basic helix-loop-helix, leucine zipper protein that forms heterodimers with MYC and MAD - regulating gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Max Antibody (NBP2-90153)(4)

Publications using NBP2-90153

• Carle K, Kellie JF, Gunn GR, Jiang Y Determination of label efficiency and label degree of critical reagents by LC-MS and native MS Analytical biochemistry 2022-12-27 [PMID: 36584741]
• Campbell GR, Rawat P, Spector SA Pacritinib Inhibition of IRAK1 Blocks Aberrant TLR8 Signalling by SARS-CoV-2 and HIV-1-Derived RNA Journal of innate immunity Jul 4 2022 [PMID: 35785771] (WB, Human)
• Cate D, Bishop J, Hsieh H et al. Antibody Screening Results for Anti-Nucleocapsid Antibodies Toward the Development of a Lateral Flow Assay to Detect SARS-CoV-2 Nucleocapsid Protein ACS Omega Sep 21 2021 [PMID: 34608447]
• Cate D, Bishop J, Hsieh H et al. Antibody Screening Results for Anti-Nucleocapsid Antibodies Toward the Development of a Lateral Flow Assay to Detect SARS-CoV-2 Nucleocapsid Protein ACS Omega Sep 21 2021 [PMID: 34608447]

Bioinformatics

• Gene Symbol: MAX