MAVS Overexpression Lysate

Western Blot: MAVS Overexpression Lysate (Adult Normal) [NBP2-06621] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAVS.

• Reactivity: Hu
• Applications: WB

MAVS Overexpression Lysate Summary

• Description:

  MAVS Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC208175
  
  Accession#: NM_020746
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: MAVS

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 56.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for MAVS Overexpression Lysate

• CARD adapter inducing interferon beta
• CARD adapter inducing interferon-beta
• CARD adaptor inducing IFN-beta
• Cardif
• DKFZp547C224
• DKFZp666M015
• FLJ27482
• IFN-B promoter stimulator 1
• Interferon beta promoter stimulator protein 1
• interferon-beta promoter stimulator protein 1
• IPS-1
• IPS-1FLJ41962
• IPS1MGC3260
• KIAA1271FLJ35386
• MAVS
• mitochondrial antiviral signaling protein
• mitochondrial antiviral-signaling protein
• mitochondrial viral signaling protein
• Putative NF-kappa-B-activating protein 031N
• virus-induced signaling adapter variant 1b
• virus-induced signaling adaptor variant 1a
• Virus-induced-signaling adapter
• VISA
• VISAFLJ38051

Background

Double-stranded RNA viruses are recognized in a cell type-dependent manner by the transmembrane receptor TLR3 (MIM 603029) or by the cytoplasmic RNA helicases MDA5 (MIM 606951) and RIGI (ROBO3; MIM 608630). These interactions initiate signaling pathways that differ in their initial steps but converge in the activation of the protein kinases IKKA (CHUK; MIM 600664) and IKKB (IKBKB; MIM 603258), which activate NFKB (see MIM 164011), or TBK1 (MIM 604834) and IKKE (IKBKE; MIM 605048), which activate IRF3 (MIM 603734). Activated IRF3 and NFKB induce transcription of IFNB (IFNB1; MIM 147640). For the TLR3 pathway, the intermediary molecule before the pathways converge is the cytoplasmic protein TRIF (TICAM1; MIM 607601). For RIGI, the intermediary protein is mitochondria-bound IPS1 (Sen and Sarkar, 2005).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

FAQs for MAVS Lysate (NBP2-06621). (Showing 1 - 1 of 1 FAQ).

1. Molecular weight of MAVS?
   • MAVS is a 540 aa protein with a predicted size of 56 kDa.

Bioinformatics

• Gene Symbol: MAVS