ZNF638 Antibody

Western Blot: ZNF638 Antibody [NB100-77291] - Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZNF638/NP220 antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Western Blot: ZNF638 Antibody [NB100-77291] - Detection of Human ZNF638/NP220 on HeLa whole cell lysate using NB100-291. ZNF638/NP220 was also immunoprecipitated by another rabbit anti-ZNF638/NP220 antibody.

Western Blot: ZNF638 Antibody [NB100-77291] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZNF638/NP220 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunoprecipitation: ZNF638 Antibody [NB100-77291] - Detection of human ZNF638/NP220 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZNF638/NP220 antibody NB100-77291 used for IP at 6 ug per reaction. ZNF638/NP220 was also immunoprecipitated by rabbit anti-ZNF638/NP220 antibody NB100-77290. For blotting immunoprecipitated ZNF638/NP220, NB100-77291 was used at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ZNF638 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1928 and 1978 of human zinc finger protein 638 (Nuclear protein 220) using the numbering given in entry NP_055312.2 (GeneID 27332).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZNF638
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZNF638 Antibody

• CTCL tumor antigen se33-1
• CTCL-associated antigen se33-1
• Cutaneous T-cell lymphoma-associated antigen se33-1
• DKFZp686P1231
• MGC26130
• MGC90196
• NP220 nuclear protein
• NP220zinc finger, matrin-like
• Nuclear protein 220
• ZFML
• Zfp638
• Zinc finger matrin-like protein
• zinc finger protein 638

Background

The protein encoded by this gene is a nucleoplasmic protein. It binds cytidine-rich sequences in double-stranded DNA. This protein has three types of domains: MH1, MH2 (repeated three times) and MH3. It is associated with packaging, transferring, or processing transcripts. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZNF638 Antibody (NB100-77291)(1)

Publication using NB100-77291

• Meruvu S, Hugendubler L, Mueller E et al. Regulation of adipocyte differentiation by the zinc finger protein ZNF638. J Biol Chem 2011-07-01 [PMID: 21602272]

Review for ZNF638 Antibody (NB100-77291) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Arunava Roy WB Human 05/14/2019

Summary

• Application: Western Blot
• Sample Tested: B cell lymphoma,Human Foreskin Fibroblasts
• Species: Human
• Lot: A3

Bioinformatics

• Gene Symbol: ZNF638
• Entrez:
  • 27332(Human)
• Uniprot:
  • Q14966(Human)