Matrin 3 Antibody - BSA Free

Immunohistochemistry: Matrin 3 Antibody [NB100-1559] - Sample: FFPE section of human laryengeal squamous cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- Matrin 3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: Matrin 3 Antibody [NB100-1559] - Detection of Human Matrin 3 by Western Blot after Immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, 1/4 of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Matrin 3 antibody NB100-1559 used for IP at 3 mcg/mg lysate. Matrin 3 was also immunoprecipitated using rabbit anti-Matrin 3 NB100-1560 and another Matrin 3 Ab at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time of 3 seconds.

• Reactivity: Hu, Mu
• Applications: IHC, IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Matrin 3 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 475 and 500 of human Matrin 3 using the numbering given in entry NP_061322.2 (GeneID 9782).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MATR3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1-4 ug/mg of lysate
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Matrin 3 Antibody - BSA Free

• distal 2
• DKFZp686K0542
• DKFZp686K23100
• matrin 3
• matrin-3
• vocal cord and pharyngeal weakness with distal myopathy

Background

Matrin 3 is a nuclear matrix protein that has been observed to mediate multiple cellular processes. Matrin 3 can function to withhold defective RNAs within the nucleus by anchoring them to the nuclear matrix and modulate the promoter activity of genes proximal to matrix/scaffold attachment region (MAR/SAR). Matrin 3 has also been demonstrated to be the main PKA substrate following NMDA receptor activation in NMDA-induced neuronal cell death. Alternate names for Matrin 3 include MATR3, MGC9105, KIAA0723, DKFZp686K0542, and DKFZp686K23100.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Matrin 3 Antibody (NB100-1559)(1)

Publication using NB100-1559

• Dunham-Ems SM, Lee YW, Stachowiak EK et al. Fibroblast growth factor receptor-1 (FGFR1) nuclear dynamics reveal a novel mechanism in transcription control. Mol Biol Cell 2009-05-01 [PMID: 19261810]

Bioinformatics

• Gene Symbol: MATR3
• Entrez:
  • 9782(Human)
  • 17184(Mouse)
• Uniprot:
  • NP_061322.2(Human)