Matrilin-2 Antibody

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Matrilin-2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Matrilin-2
  Arg24-Arg956
  Accession # AAH05429
• Specificity: Detects mouse Matrilin-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human Matrilin-2 is observed and less than 5% cross-reactivity with recombinant mouse (rm) Matrilin-3 and rmMatrilin-4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MATN2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Matrilin-2 Antibody

• MATN2
• matrilin 2
• Matrilin2
• Matrilin-2

Background

Matrilin-2 is an extracellular matrix protein that belongs to the superfamily of von Willebrand factor A domain (VWA) containing proteins. It is expressed in many tissues and functions as a bridging component between other matrix molecules (1, 2, 3, 4). The mouse Matrilin‑2 cDNA encodes a 956 amino acid (aa) precursor with a 23 aa signal sequence, two VWA domains separated by ten tandem EGF-like repeats, and a C‑terminal coiled coil domain (5). Mouse Matrilin-2 shares 84%‑87% aa sequence identity with human, rat, and canine Matrilin-2, and 26%, 21%, and 34% aa sequence identity with mouse Matrilin-1, -3, and -4, respectively. Matrilin-2 forms a variety of disulfide-linked oligomers via its coiled coil domain (4, 6‑8). It can assemble into homotrimers or heterotrimers with Matrilin-1 and/or Matrilin-4 (4, 6, 7) but has not been detected in heterotrimers containing Matrilin-3 (7). The VWA domains are thought to mediate Matrilin-Matrilin interactions as well as interactions with other matrix proteins such as Fibronectin, Collagen I, Fibrilin-2, and Laminin-1/Nidogen-1 complexes (6). Matrilin-2 knockout mice do not display any obvious abnormalities, suggesting that the expression of other molecules can compensate for the lack of Matrilin-2 (9).

1. Wagener, R. et al. (2005) FEBS Lett. 579:3323.
2. Deak, F. et al. (1999) Matrix Biol. 18:55.
3. Whittaker, C.A. and R.O. Hynes (2002) Mol. Biol. Cell 13:3369.
4. Piecha, D. et al. (1999) J. Biol. Chem. 274:13353.
5. Deak, F. et al. (1997) J. Biol. Chem. 272:9268.
6. Piecha, D. et al. (2002) Biochem. J. 367:715.
7. Frank, S. et al. (2002) J. Biol. Chem. 277:19071.
8. Pan, O.H. and K. Beck (1998) J. Biol. Chem. 273:14205.
9. Mates, L. et al. (2004) Matrix Biol. 23:195.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Matrilin-2 Antibody (AF3234)(1)

Publication using AF3234

• S Eg, S Rosenzweig, D DiTullio, JD Hinman, SP Bridges, MA Marin, R Kawaguchi, G Coppola, ST Carmichael White matter stroke induces a unique oligo-astrocyte niche that inhibits recovery J. Neurosci., 2019-10-07;0(0):. 2019-10-07 [PMID: 31591156] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: MATN2
• Uniprot:
  • AAH05429()