MATE1 Antibody

Western Blot: MATE1 Antibody [NBP1-36989] - Staining of Human Liver lysate (35 ug protein in RIPA buffer) with antibody at 0.1 ug/mL. Detected by chemiluminescence. Approx. 60-65kDa band observed in Human Liver lysates (calculated MW of 61.9 kDa according to NP_060712.2). This band was successfully blocked by incubation with the immunizing peptide.

Immunocytochemistry/Immunofluorescence: MATE1 Antibody [NBP1-36989] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL). Strong expression of the protein seen in the plasma membrane of HepG2 cells.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MATE1 Antibody Summary

• Immunogen: This MATE1 Antibody was developed against a peptide with sequence C-PEHPQDGAKLSRKQ corresponding to internal region (near C-Terminus) according to NP_060712.2.
• Epitope: internal region (near C-Terminus)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SLC47A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 0.3 ug/mL

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MATE1 Antibody

• hMATE-1
• MATE1
• MATE-1
• MATE1FLJ10847multidrug and toxin extrusion 1
• MGC64822
• multidrug and toxin extrusion protein 1
• SLC47A1
• Solute carrier family 47 member 1
• solute carrier family 47, member 1

Background

The MATE1 gene is located within the Smith-Magenis syndrome region on chromosome 17. It encodes a protein of unknown function. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MATE1 Antibody (NBP1-36989)(1)

Publication using NBP1-36989

• Chen Y, Zhang S, Sorani M, Giacomini KM. Transport of paraquat by human organic cation transporters and multidrug and toxic compound extrusion family. J Pharmacol Exp Ther 2007-08-01 [PMID: 17495125]

Bioinformatics

• Gene Symbol: SLC47A1
• Entrez:
  • 55244(Human)
• Uniprot:
  • NP_060712.2(Human)