MARK2 Antibody (3B12)

Western Blot: MARK2 Antibody (3B12) [H00002011-M01] - MARK2 monoclonal antibody (M01), clone 3B12. Analysis of MARK2 expression in NIH/3T3.

Western Blot: MARK2 Antibody (3B12) [H00002011-M01] - MARK2 monoclonal antibody (M01), clone 3B12. Analysis of MARK2 expression in PC-12.

Western Blot: MARK2 Antibody (3B12) [H00002011-M01] - MARK2 monoclonal antibody (M01), clone 3B12. Analysis of MARK2 expression in A-431.

ELISA: MARK2 Antibody (3B12) [H00002011-M01] - Detection limit for recombinant GST tagged MARK2 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clone: 3B12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MARK2 Antibody (3B12) Summary

• Immunogen: MARK2 (AAH08771, 401 a.a. ~ 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QAAGPAIPTSNSYSKKTQSNNAENKRPEEDRESGRKASSTAKVPASPLPGLERKKTTPTPSTNSVLSTSTNRSRNSPLLERASLGQASIQNGKDSLTMPG
• Specificity: MARK2 - MAP/microtubule affinity-regulating kinase 2
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MARK2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Western Blot 1:500-1:1000
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 29941476).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MARK2 Antibody (3B12)

• EC 2.7.11
• EC 2.7.11.1
• ELKL motif kinase 1
• ELKL motif kinase
• EMK-1
• EMK1PAR-1
• MAP/microtubule affinity-regulating kinase 2MGC99619
• PAR1 homolog
• Par1b
• Ser/Thr protein kinase PAR-1B
• serine/threonine protein kinase EMK
• serine/threonine-protein kinase MARK2

Background

EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1. These isoforms were characterized by the presence of a 162-bp alternative exon that gave rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be coexpressed in a number of selected cell lines and tissue samples. The human EMK1 was shown to be encoded by a single mRNA ubiquitously expressed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MARK2 Antibody (H00002011-M01)(3)

Publications using H00002011-M01

• Hart M, Zulkipli I, Shrestha RL et al. MARK2/Par1b kinase present at centrosomes and retraction fibres corrects spindle off-centring induced by actin disassembly Open Biol 2019-06-28 [PMID: 31238822] (WB, Human)
• Zulkipli I, Clark J, Hart M et al. Spindle rotation in human cells is reliant on a MARk2-mediated equatorial spindle-centering mechanism. J. Cell Biol. 2018-06-25 [PMID: 29941476] (ICC/IF, Human)
• Hezel AF, Gurumurthy S, Granot Z et al. Pancreatic Lkb1 Deletion Leads to Acinar Polarity Defects Cystic Neoplasms. Mol Cell Biol;28(7):2414-25. 2008-04-01 [PMID: 18227155]

FAQs for MARK2 Antibody (H00002011-M01). (Showing 1 - 1 of 1 FAQs).

1. I am interested in MARK2 antibody (3B12), and I would like to know how you have verified its specificity. Did you block with antigen peptide? Or, has it been verified in transfected cells, knockout cell, or siRNA cells?
   • The MARK2 antibody was validated in the following cell lysates: PC-12, A-431 and NIH/3T3 as well as validated against recombinant protein/immunogen.

Bioinformatics

• Gene Symbol: MARK2
• Entrez:
  • 2011(Human)
• OMIM:
  • 600526(Human)
• Uniprot:
  • AAH08771(Human)