MARCO Antibody (579511) [Fluorescein]

Mouse splenocytes were stained with Rat Anti-Mouse MARCO Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2956F, filled histogram) or isotype control antibody (Catalog # IC005F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Mouse splenocytes were differentiated to dendritic cells by culturing with recombinant mouse IL-4 (Catalog # 404-ML, 20 ng/ml) and recombinant mouse GM-CSF (Catalog # 415-ML, 40 ng/ml) for 6 days, then stained with (A) Rat Anti-Mouse MARCO Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2956F) or (B) isotype control antibody (Catalog # IC005F) and Rat anti-Mouse CD11c Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB69501R). View our protocol for Staining Membrane-associated Proteins.

J774 mouse monocyte-macrophage cell line, resting (open histogram), or treated with 500 ng/ml LPS for 3 days (filled histogram) were stained with Rat Anti-Mouse MARCO CFS-conjugated Monoclonal Antibody (Catalog # FAB2956F). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 579511
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Fluorescein

MARCO Antibody (579511) [Fluorescein] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MARCO
  Gln70-Ser518
  Accession # Q60754
• Specificity: Detects mouse MARCO in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: MARCO
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  12 months from date of receipt, 2 to 8 degreesC as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MARCO Antibody (579511) [Fluorescein]

• macrophage receptor with collagenous structureSCARA2Scavenger receptor class A member 2
• MARCO
• member 2
• SCARA2

Background

MARCO (macrophage receptor with collagenous structure), also known as SCARA2, is an 80 kDa type II transmembrane glycoprotein that belongs to the class A scavenger receptor family (1). Mouse MARCO consists of a 48 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 449 aa extracellular domain (ECD) that includes a stalk region, a collagen-like region, and one SRCR domain (2). Within the ECD, mouse MARCO shares 69% and 86% aa sequence identity with human and rat MARCO, respectively. It shares 18%-28% aa sequence identity with other mouse class A scavenger receptors CL-P1, SCARA3, SCARA5, and SR-A1/MSR. MARCO is constitutively expressed on the surface of splenic and lymph node macrophages (2, 3). Its expression is induced on Kupffer cells and alveolar macrophages by microbial infection, chemical irritants, and Th1 polarizing factors (3-5). MARCO binds LPS, lipoteichoic acid, and other determinants on Gram positive and Gram negative bacteria (2, 6-8). It also binds modified LDL, CpG oligonucleotides, UGRP1, silica, and TiO 2 (2, 9-11). MARCO is required for the organization of the splenic marginal zone and the interaction of local macrophages and B cells (12, 13). The SRCR domain mediates binding of MARCO to its various ligands (3, 12), while the collagen-like region mediates assembly into a disulfide-linked trimeric molecule (2, 7). MARCO ligation induces, but is not required for the production of IL-12, NO, or TNF-alpha by macrophages (5, 6, 9). MARCO knockout mice show a reduced clearance of bacterial infections, reduced mast cell mediated silicosis, increased pulmonary inflammation, and increased sensitivity to ozone induced lung damage (4, 9, 14-16).

1. Murphy, J.E. et al. (2005) Atherosclerosis 182:1.
2. Elomaa, O. et al. (1995) Cell 80:603.
3. Van der Laan, L.J.W. et al. (1999) J. Immunol. 162:939.
4. Dahl, M. et al. (2007) J. Clin. Invest. 117:757.
5. Jozefowski, S. et al. (2005) J. Immunol. 175:8032. 
6. Mukhopadhyay, S. et al. (2006) Eur. J. Immunol. 36:940. 
7. Sankala, M. et al. (2002) J. Biol. Chem. 277:33378. 
8. Chen, Y. et al. (2006) J. Biol. Chem. 281:12767. 
9. Jozefowski, S. et al. (2006) J. Leukoc. Biol. 80:870.
10. Bin, L.-H. et al. (2003) J. Immunol. 171:924.
11. Hamilton, Jr. R.F. et al. (2006) J. Biol. Chem. 281:34218.
12. Karlsson, M.C.I. et al. (2003) J. Exp. Med. 198:333.
13. Chen, Y. et al. (2005) J. Immunol. 175:8173.
14. Arredouani, M. et al. (2004) J. Exp. Med. 200:267.
15. Arredouani, M.S. et al. (2007) J. Immunol. 178:5912.
16. Brown, J.M. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:43.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MARCO Antibody (FAB2956F)(3)

Publications using FAB2956F

• Onyishi, CU;Desanti, GE;Wilkinson, AL;Lara-Reyna, S;Frickel, EM;Fejer, G;Christophe, OD;Bryant, CE;Mukhopadhyay, S;Gordon, S;May, RC; Toll-like receptor 4 and macrophage scavenger receptor 1 crosstalk regulates phagocytosis of a fungal pathogen Nature communications 2023-08-14 [PMID: 37580395] (Flow Cytometry, Mouse)
• Véronique Collin-Faure, Marianne Vitipon, Anaëlle Torres, Ornella Tanyeres, Bastien Dalzon, Thierry Rabilloud The internal dose makes the poison: higher internalization of polystyrene particles induce increased perturbation of macrophages Frontiers in Immunology 5/12/2023 [PMID: 37251378]
• Y Vagima, D Gur, N Erez, H Achdout, M Aftalion, Y Levy, A Zauberman, A Tidhar, H Gutman, S Lazar, T Israely, N Paran, S Melamed, T Brosh-Niss, T Chitlaru, I Sagi, E Mamroud Influenza virus infection augments susceptibility to respiratory Yersinia pestis exposure and impacts the efficacy of antiplague antibiotic treatments Sci Rep, 2020-11-05;10(1):19116. 2020-11-05 [PMID: 33154422] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: MARCO
• Uniprot:
  • Q60754()