MAP4K5 Antibody (3H3)

Images

 
Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01] - Analysis of MAP4K5 expression in transfected 293T cell line by MAP4K5 monoclonal antibody (M01), clone 3H3.Lane 1: MAP4K5 transfected lysate(95.04 KDa).Lane 2: ...read more
Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01] - Western Blot analysis of MAP4K5 expression in 293 ( Cat # L026V1 ).
Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01] - Analysis of MAP4K5 over-expressed 293 cell line, cotransfected with MAP4K5 Validated Chimera RNAi ( Cat # H00011183-R01V ) (Lane 2) or non-transfected control (Lane ...read more
ELISA: MAP4K5 Antibody (3H3) [H00011183-M01] - Detection limit for recombinant GST tagged MAP4K5 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
3H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

MAP4K5 Antibody (3H3) Summary

Immunogen
MAP4K5 (AAH36013, 361 a.a. ~ 470 a.a) partial recombinant protein with GST tag.SSDPNFMLQWNPFVDGANTGKSTSKRAIPPPLPPKPRISSYPEDNFPDEEKASTIKHCPDSESRAPQILRRQSSPSCGPVAETSSIGNGDGISKLMSENTEGSAQAPQLP
Specificity
MAP4K5 - mitogen-activated protein kinase kinase kinase kinase 5
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MAP4K5
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MAP4K5 Antibody (3H3)

  • EC 2.7.11
  • EC 2.7.11.1
  • GCKR
  • germinal center kinase-related
  • KHS1
  • KHSMEKKK 5
  • Kinase homologous to SPS1/STE20
  • MAP4K5
  • MAPK/ERK kinase kinase kinase 5
  • MAPKKKK5
  • MEK kinase kinase 5
  • mitogen-activated protein kinase kinase kinase kinase 5

Background

This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAP4K5
Entrez
OMIM
Uniprot