MANEA Antibody - BSA Free

Western Blot: MANEA Antibody - BSA Free [NBP3-45962] - HEK-293 cells were subjected to SDS PAGE followed by western blot with ( MANEA Antibody) at dilution of 1:300

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MANEA Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MANEA Antibody - BSA Free (NBP3-45962) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: mannosidase, endo-alpha. (Uniprot# Q5SRI9)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MANEA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500-1:2000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MANEA Antibody - BSA Free

• alpha 1,2-endomannosidase
• DKFZp686D20120
• EC 3.2.1
• EC 3.2.1.130
• ENDO
• Endo-alpha mannosidase
• endomannosidase
• FLJ12838
• glycoprotein endo-alpha-1,2-mannosidase
• hEndo
• mandaselin
• mannosidase, endo-alpha

Background

N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MANEA