MAGEA4 Antibody (OTI2C1) - Azide and BSA Free

Western Blot: MAGEA4 Antibody (OTI2C1) - Azide and BSA Free [NBP2-72571] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAGEA4.

Immunocytochemistry/Immunofluorescence: MAGEA4 Antibody (OTI2C1) - Azide and BSA Free [NBP2-72571] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MAGEA4.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: OTI2C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

MAGEA4 Antibody (OTI2C1) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human MAGEA4(NP_001011550) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAGEA4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Theoretical MW: 34.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for MAGEA4 Antibody (OTI2C1) - Azide and BSA Free

• CT1.4
• MAGE-41
• MAGE4A
• MAGE4B
• MAGEA4
• MAGE-X2 antigen
• MAGE-X2
• melanoma antigen family A, 4
• melanoma-associated antigen 4
• member 4

Background

MAGEA4 is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAGEA4