MAGEA2 Antibody (1H4) - Azide and BSA Free

Western Blot: MAGEA2 Antibody (1H4) [H00004101-M01] - Analysis of MAGEA2 expression in transfected 293T cell line by MAGEA2 monoclonal antibody (M01), clone 1H4.Lane 1: MAGEA2 transfected lysate(35 KDa).Lane 2: Non-transfected lysate.

Western Blot: MAGEA2 Antibody (1H4) [H00004101-M01] - MAGEA2 monoclonal antibody (M01), clone 1H4 Analysis of MAGEA2 expression in Jurkat.

Sandwich ELISA: MAGEA2 Antibody (1H4) [H00004101-M01] - Detection limit for recombinant GST tagged MAGEA2 is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1H4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MAGEA2 Antibody (1H4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MAGEA2 (AAH63681, 1 a.a. ~ 314 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPLEQRSQHCKPEEGLEARGEALGLVGAQAPATEEQQTASSSSTLVEVTLGEVPAADSPSPPHSPQGASSFSTTINYTLWRQSDEGSSNQEEEGPRMFPDLESEFQAAISRKMVELVHFLLLKYQAREPVTKAEMLESVLRNCQDFFPVIFSKASEYLQLVFGIEVVEVVPISHLYILVTCLGLSYDGLLGDNQVMPKTGLLIIVLAIIAIEGDCAPEEKIWEELSMLEVFEGREDSVFAHPRKLLMQDLVQENYLEYRQVPGSDPACYEFLWGPRALIETSYVKVLHHTLKIGGEPHISYPPLHERALREGEE
• Specificity: MAGEA2 - melanoma antigen family A, 2
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAGEA2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MAGEA2 Antibody (1H4) - Azide and BSA Free

• Cancer/testis antigen 1.2
• CT1.2MGC131923
• MAGE-2 antigen
• MAGEA2Amember 2
• MAGEA2B
• melanoma antigen 2
• melanoma antigen family A, 2
• melanoma-associated antigen 2

Background

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci. Alternatively spliced transcript variants encoding the same protein have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAGEA2
• Entrez:
  • 4101(Human)
• OMIM:
  • 300173(Human)
• Uniprot:
  • AAH63681(Human)