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MAGEA2 Antibody (1B1)

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Immunocytochemistry/Immunofluorescence: MAGEA2 Antibody (1B1) [H00004101-M02] - Analysis of monoclonal antibody to MAGEA2 on HeLa cell. Antibody concentration 20 ug/ml.
Immunocytochemistry/ Immunofluorescence: MAGEA2 Antibody (1B1) [H00004101-M02] - Analysis of monoclonal antibody to MAGEA2 on HeLa cell. Antibody concentration 20 ug/ml.
Sandwich ELISA: MAGEA2 Antibody (1B1) [H00004101-M02] - Detection limit for recombinant GST tagged MAGEA2 is 1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
1B1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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MAGEA2 Antibody (1B1) Summary

Immunogen
MAGEA2 (AAH63681, 1 a.a. - 314 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPLEQRSQHCKPEEGLEARGEALGLVGAQAPATEEQQTASSSSTLVEVTLGEVPAADSPSPPHSPQGASSFSTTINYTLWRQSDEGSSNQEEEGPRMFPDLESEFQAAISRKMVELVHFLLLKYQAREPVTKAEMLESVLRNCQDFFPVIFSKASEYLQLVFGIEVVEVVPISHLYILVTCLGLSYDGLLGDNQVMPKTGLLIIVLAIIAIEGDCAPEEKIWEELSMLEVFEGREDSVFAHPRKLLMQDLVQENYLEYRQVPGSDPACYEFLWGPRALIETSYVKVLHHTLKIGGEPHISYPPLHERALREGEE
Specificity
MAGEA2 - melanoma antigen family A, 2 (1B1)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MAGEA2
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MAGEA2 Antibody (1B1)

  • Cancer/testis antigen 1.2
  • CT1.2MGC131923
  • MAGE-2 antigen
  • MAGEA2Amember 2
  • MAGEA2B
  • melanoma antigen 2
  • melanoma antigen family A, 2
  • melanoma-associated antigen 2

Background

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci. Alternatively spliced transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00005670-Q01
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Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
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Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in red, and costained with chicken pAb to Microtubule Associated Protein2 (MAP2), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. DCX antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while MAP2 antibody stains dendrites and perikarya of mature neurons. Therefore DCX antibody is an excellent marker of developing neuronal cells.
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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Western Blot: MAGEA4 Antibody [NBP2-41318] - Analysis of MAGEA4 in A431 cell lysate with MAGEA4 antibody at 1 ug/ml.
Immunocytochemistry: MAGEA4 Antibody [NBP2-41318] - Staining of MAGEA4 in HeLa cells with MAGEA4 antibody at 5 ug/ml.
Immunohistochemistry-Paraffin: MAGEA4 Antibody [NBP2-41318] - Human breast tumor with MAGEA4 antibody at 5 ug/mL.
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(5 Reviews)
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NB500-201
Western Blot: Survivin Antibody [NB500-201] - Whole cell extracts were prepared in RIPA buffer and analyzed using immunoblot analysis. PVDF membrane. Primary antibody 1:1000 at 4C overnight. Secondary antibody 1:20000 at 4C for 1 hour. This image was submitted via customer Review.
Immunocytochemistry/Immunofluorescence: Survivin Antibody [NB500-201] - Analysis using the HRP conjugate of NB500-201. Staining of Telophase with accumulation of survivin in the midbodies of two daughter cells. Survivin detection using NB500-201.
Immunohistochemistry-Paraffin: Survivin Antibody [NB500-201] - Survivin shows lysates of human neuroblastoma cell line. PVDF membrane was probed with 1:200 dilution of 0.5 ug/mL of rabbit anti-Survivin polyclonal (NB500-201, Novus Biologicals), followed by 1:2000 dilution of goat anti-rabbit IgG.
(20 Reviews)
(295 Publications)
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MAGEA2 Antibody
(1B1)
H00004101-M02
MAGEA2 Antibody (1B1)
Species: Hu
Applications: WB, ELISA, ICC/IF

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Bioinformatics

Gene Symbol MAGEA2
Entrez
Uniprot