MafG Antibody (370412)

Western blot shows recombinant human MafF, MafG, and MafK (5 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human MafG Monoclonal Antibody (Catalog # MAB3924) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for MafG was detected at approximately 18 kDa. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human MafG Monoclonal Antibody (Catalog # MAB3924) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for MafG was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 370412
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MafG Antibody (370412) Summary

• Immunogen: E. coli-derived recombinant human MafG
  Met1-Ser162
  Accession # O15525
• Specificity: Detects human MafG in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MafF or rhMafK is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAFG
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MafG Antibody (370412)

• basic leucine zipper transcription factor MafG
• hMAF
• MafG
• MGC13090
• MGC20149
• transcription factor MafG
• v-maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein G
• v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian)
• V-maf musculoaponeurotic fibrosarcoma oncogene homolog G

Background

Maf family members form a unique subclass of basic-leucine zipper (bZIP) transcription factors. Maf proteins are subdivided into two groupings: large, including c-Maf, Nrl, MafA, and MafB; and small, including MafF, MafG, and MafK. Large Mafs contain an N-terminal acidic domain important for transcriptional activation that is lacking in small Maf family members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MafG Antibody (MAB3924)(1)

Publication using MAB3924

• Faiza Noreen, Taya Küng, Luigi Tornillo, Hannah Parker, Miguel Silva, Stefan Weis et al. DNA methylation instability by BRAF-mediated TET silencing and lifestyle-exposure divides colon cancer pathways Clinical Epigenetics 2019-12-16 [PMID: 31842975]

Bioinformatics

• Gene Symbol: MAFG
• Uniprot:
  • O15525()