MAD2L2 Antibody (JU99-23)

Western Blot: MAD2L2 Antibody (JU99-23) [NBP2-75562] - Analysis of MAD2L2 on K562 cell lysates with Rabbit anti-Mad2L2 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: MAD2L2 Antibody (JU99-23) [NBP2-75562] - Analysis of paraffin-embedded rat lung tissue using anti-Mad2L2 antibody. Counter stained with hematoxylin.

Flow Cytometry: MAD2L2 Antibody (JU99-23) [NBP2-75562] - Analysis of Hela cells with Mad2L2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: MAD2L2 Antibody (JU99-23) [NBP2-75562] - Analysis of Mad2L2 on SiHa cell using anti-Mad2L2 antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: MAD2L2 Antibody (JU99-23) [NBP2-75562] - Analysis of paraffin-embedded human tonsil tissue using anti-Mad2L2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: MAD2L2 Antibody (JU99-23) [NBP2-75562] - Analysis of paraffin-embedded mouse colon tissue using anti-Mad2L2 antibody. Counter stained with hematoxylin.

Product Discontinued

MAD2L2 Antibody (JU99-23) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human MAD2L2 aa 162-211 / 211. (SwissProt: Q9UI95 Human; SwissProt: Q9D752 Mouse; SwissProt: D3Z8D9 Rat)
• Localization: Nucleus. Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAD2L2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MAD2L2 Antibody (JU99-23)

• hREV7
• MAD2 mitotic arrest deficient-like 2 (yeast)
• MAD2BREV7 homolog
• MAD2-like protein 2
• Mitotic arrest deficient 2-like protein 2
• mitotic arrest deficient homolog-like 2
• mitotic spindle assembly checkpoint protein MAD2B
• REV7yeast, homolog)-like 2

Background

MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAD2L2