MAD2L2 Antibodies
We offer MAD2L2 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each MAD2L2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our MAD2L2 Antibodies can be used in a variety of model species: Human. Use the list below to choose the MAD2L2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our MAD2L2 polyclonal antibodies.
Alternate Names for MAD2L2 Antibodies
anti-MAD2L2 antibody, anti-hREV7 antibody, anti-MAD2 mitotic arrest deficient-like 2 (yeast) antibody, anti-MAD2-like protein 2 antibody, anti-MAD2BREV7 homolog antibody, anti-Mitotic arrest deficient 2-like protein 2 antibody, anti-mitotic arrest deficient homolog-like 2 antibody, anti-mitotic spindle assembly checkpoint protein MAD2B antibody, anti-REV7yeast, homolog)-like 2 antibody
11 Results for "mad2l2" in Primary Antibodies
![Western Blot: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Western blot analysis of extracts of various cell lines, using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-7B6I2-Western-Blot-NBP3-16451-img0006.jpg "Western Blot: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Western blot analysis of extracts of various cell lines, using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Immunofluorescence analysis of NIH-3T3 cells using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-7B6I2-Immunocytochemistry-Immunofluorescence-NBP3-16451-img0005.jpg)
![Immunohistochemistry-Paraffin: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Immunohistochemistry of paraffin-embedded mouse brain using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-7B6I2-Immunohistochemistry-Paraffin-NBP3-16451-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Immunofluorescence analysis of C6 cells using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-7B6I2-Immunocytochemistry-Immunofluorescence-NBP3-16451-img0004.jpg)
![Immunohistochemistry-Paraffin: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Immunohistochemistry of paraffin-embedded rat brain using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-7B6I2-Immunohistochemistry-Paraffin-NBP3-16451-img0001.jpg)
![Immunohistochemistry-Paraffin: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Immunohistochemistry of paraffin-embedded human colon using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-7B6I2-Immunohistochemistry-Paraffin-NBP3-16451-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: MAD2L2 Antibody [NBP3-35251] - Immunofluorescence analysis of L929 cells using MAD2L2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35251_rabbit-mad2l2-pab-1012202411592885.jpg "Immunocytochemistry/ Immunofluorescence: MAD2L2 Antibody [NBP3-35251] - Immunofluorescence analysis of L929 cells using MAD2L2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Western Blot: MAD2L2 Antibody [NBP3-35251] - Western blot analysis of various lysates using MAD2L2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-35251_rabbit-mad2l2-pab-10122024120615.jpg)
![Immunocytochemistry/ Immunofluorescence: MAD2L2 Antibody [NBP3-35251] - Immunofluorescence analysis of U2OS cells using MAD2L2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35251_rabbit-mad2l2-pab-101220241243958.jpg)
![Immunocytochemistry/ Immunofluorescence: MAD2L2 Antibody [NBP3-35251] - Immunofluorescence analysis of H9C2 cells using MAD2L2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35251_rabbit-mad2l2-pab-101220241252827.jpg)
![Western Blot: MAD2L2 Antibody [H00010459-D01P] - Analysis of MAD2L2 expression in transfected 293T cell line by MAD2L2 polyclonal antibody.Lane 1: MAD2L2 transfected lysate(24.30 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-Western-Blot-H00010459-D01P-img0002.jpg "Western Blot: MAD2L2 Antibody [H00010459-D01P] - Analysis of MAD2L2 expression in transfected 293T cell line by MAD2L2 polyclonal antibody.Lane 1: MAD2L2 transfected lysate(24.30 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry: MAD2L2 Antibody (4D8) [NBP3-26158] - Image of MAD2L2 Antibody (4D8) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26158_rabbit-mad2l2-mab-4d8-27220248313714.jpg "Immunohistochemistry: MAD2L2 Antibody (4D8) [NBP3-26158] - Image of MAD2L2 Antibody (4D8) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: MAD2L2 Antibody (4D8) [NBP3-26158] - Positive Western Blot detected in: 293 whole cell lysate. All lanes: MAD2L2 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 25 kDa Observed band size: 25 kDa](http://images.novusbio.com/fullsize/nbp3-26158_rabbit-mad2l2-mab-4d8-28220241140925.jpg)
![Immunocytochemistry/Immunofluorescence: MAD2L2 Antibody (4D8) [NBP3-26158] - Staining of Hela Cells with MAD2L2 Antibody (4D8) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26158_rabbit-mad2l2-mab-4d8-282202412475128.jpg)
![Western Blot: MAD2L2 Antibody [NBP1-02798] - Affinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a ~20 kD band corresponding to human MAD2L2 (predicted molecular weight is 24 kD). Approximately 20 ug of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-Western-Blot-NBP1-02798-img0003.jpg "Western Blot: MAD2L2 Antibody [NBP1-02798] - Affinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a ~20 kD band corresponding to human MAD2L2 (predicted molecular weight is 24 kD). Approximately 20 ug of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.")
![Immunohistochemistry-Paraffin: MAD2L2 Antibody [NBP1-02798] - Staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-Immunohistochemistry-Paraffin-NBP1-02798-img0001.jpg)
![Western Blot: MAD2L2 Antibody [NBP3-17735] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil](http://images.novusbio.com/fullsize/MAD2L2-Antibody-Western-Blot-NBP3-17735-img0003.jpg "Western Blot: MAD2L2 Antibody [NBP3-17735] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil")
![Immunocytochemistry/Immunofluorescence: MAD2L2 Antibody [NBP3-17735] - Staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17735-img0002.jpg)
![Western Blot: MAD2L2 Antibody [NBP3-38622] - Western blot analysis of various lysates using MAD2L2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-38622_rabbit-mad2l2-pab-912202414201332.jpg "Western Blot: MAD2L2 Antibody [NBP3-38622] - Western blot analysis of various lysates using MAD2L2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.")
![Immunocytochemistry/Immunofluorescence: MAD2L2 Antibody [NBP2-58605] - Staining of human cell line U-2 OS shows localization to nucleoli & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58605-img0001.jpg "Immunocytochemistry/Immunofluorescence: MAD2L2 Antibody [NBP2-58605] - Staining of human cell line U-2 OS shows localization to nucleoli & cytosol. Antibody staining is shown in green.")
![Western Blot: MAD2L2 Antibody [NBP3-12688] - Lane A: K562 Whole Cell LysateLane B: SW480 Whole Cell LysateLane C: Hela Whole Cell LysateLane D: Molt-4 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:24 kDaObserved band size:24 kDa](http://images.novusbio.com/fullsize/MAD2L2-Antibody-Western-Blot-NBP3-12688-img0001.jpg "Western Blot: MAD2L2 Antibody [NBP3-12688] - Lane A: K562 Whole Cell LysateLane B: SW480 Whole Cell LysateLane C: Hela Whole Cell LysateLane D: Molt-4 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:24 kDaObserved band size:24 kDa")
![Western Blot: MAD2L2 Antibody [H00010459-B02P] - Analysis of MAD2L2 expression in transfected 293T cell line by MAD2L2 polyclonal antibody. Lane 1: MAD2L2 transfected lysate(23.21 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-Western-Blot-H00010459-B02P-img0002.jpg "Western Blot: MAD2L2 Antibody [H00010459-B02P] - Analysis of MAD2L2 expression in transfected 293T cell line by MAD2L2 polyclonal antibody. Lane 1: MAD2L2 transfected lysate(23.21 KDa). Lane 2: Non-transfected lysate.")