MAD2L1 Antibody [Alexa Fluor® 750]

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 750

MAD2L1 Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human MAD2L1
  Met1-Asp205
  Accession # Q13257
• Specificity: Detects human and rat MAD2L1 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MAD2L1 Antibody [Alexa Fluor® 750]

• HSMAD2
• MAD2 (mitotic arrest deficient, yeast, homolog)-like 1
• MAD2 mitotic arrest deficient-like 1 (yeast)
• MAD2L1
• MAD2-like protein 1
• MAD2mitotic arrest deficient, yeast, homolog-like 1
• Mitotic arrest deficient 2-like protein 1
• mitotic spindle assembly checkpoint protein MAD2A

Background

MAD2L1 (Mitotic arrest deficient protein 2) is a component of the spindle-attachment checkpoint mechanism that monitors kinetochore spindle attachment and leads to the subsequent arrest in early metaphase by its recruitment to unattached kinetochores. The transition from metaphase to anaphase requires the association of the anaphase promoting complex/cyclosome (APC/C) with Cdc20 leading to the ubiquitylation and subsequent degradation of Pds1/Securin. This transition is delayed by the inhibitory association of MAD2L1 with Cdc20. MAD2L1 has also been shown to be a direct E2F target and as such is aberrantly expressed in cells with retinoblastoma pathway defects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.