MAD1L1/MAD1 Overexpression Lysate (Native)

Images

 
Western Blot: MAD1 Overexpression Lysate (Adult Normal) [NBP2-11009] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAD1.

Product Details

Summary
Applications WB

Order Details

MAD1L1/MAD1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_001013859. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens MAD1, mRNA.
Gene
MAD1L1

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
RIPA buffer

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
82.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for MAD1L1/MAD1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MAD1L1/MAD1 Overexpression Lysate (Native)

  • hMAD1
  • HsMAD1
  • MAD1 mitotic arrest deficient-like 1 (yeast)
  • MAD1
  • MAD1L1
  • MAD1-like protein 1
  • MAD1yeast, homolog-like 1
  • Mitotic checkpoint MAD1 protein homolog
  • PIG9
  • Tax-binding protein 181
  • TP53I9
  • tumor protein p53 inducible protein 9
  • TXBP181
  • TXBP181MAD1 (mitotic arrest deficient, yeast, homolog)-like 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-563
Species: Hu
Applications: WB (-), ICC/IF, IHC, IHC-P, IP
NB600-302
Species: Hu, Mu, Dr
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF4185
Species: Hu
Applications: ICC
NBP1-89979
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-88624
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-24509
Species: Hu, Mu, Rt, Bv, Ca, Pm
Applications: WB, IHC-P
NB100-59828
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP, PLA
AF6028
Species: Hu
Applications: WB, IHC
H00000699-D01P
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, PLA
NBP1-77836
Species: Hu, Mu, Rt, Po, Bv, Ch, Xp, Ze
Applications: WB, ELISA, Flow, IHC, IHC-P
NB100-56507
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP
NBP1-88517
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-1116
Species: Hu
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA
NB100-1103
Species: Hu
Applications: WB, PEP-ELISA
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-81765
Species: Hu
Applications: IHC, IHC-P
NB100-353
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IP
NBP2-19957
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-317
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-11009
Applications: WB

Publications for MAD1L1/MAD1 Lysate (NBP2-11009) (0)

There are no publications for MAD1L1/MAD1 Lysate (NBP2-11009).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MAD1L1/MAD1 Lysate (NBP2-11009) (0)

There are no reviews for MAD1L1/MAD1 Lysate (NBP2-11009). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MAD1L1/MAD1 Lysate (NBP2-11009) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional MAD1L1/MAD1 Products

Related Products by Gene

Bioinformatics Tool for MAD1L1/MAD1 Lysate (NBP2-11009)

Discover related pathways, diseases and genes to MAD1L1/MAD1 Lysate (NBP2-11009). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MAD1L1/MAD1 Lysate (NBP2-11009)

Discover more about diseases related to MAD1L1/MAD1 Lysate (NBP2-11009).
 

Pathways for MAD1L1/MAD1 Lysate (NBP2-11009)

View related products by pathway.

PTMs for MAD1L1/MAD1 Lysate (NBP2-11009)

Learn more about PTMs related to MAD1L1/MAD1 Lysate (NBP2-11009).

Blogs on MAD1L1/MAD1

There are no specific blogs for MAD1L1/MAD1, but you can read our latest blog posts.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol MAD1L1
Entrez
Uniprot

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP2-11009 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought