MAD1L1/MAD1 Antibody

Product Discontinued

MAD1L1/MAD1 Antibody Summary

• Immunogen: Recombinant MAD1 protein
• Specificity: NB500-136 reacts with MAD1 where a band is seen at ~80 to 90 kDa representing MAD1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAD1L1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Western blotting 1:1,000 (ECL) Immunoprecipitation 1:50
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human and mouse. Other species have not been tested.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for MAD1L1/MAD1 Antibody

• hMAD1
• HsMAD1
• MAD1 mitotic arrest deficient-like 1 (yeast)
• MAD1
• MAD1L1
• MAD1-like protein 1
• MAD1yeast, homolog-like 1
• Mitotic checkpoint MAD1 protein homolog
• PIG9
• Tax-binding protein 181
• TP53I9
• tumor protein p53 inducible protein 9
• TXBP181
• TXBP181MAD1 (mitotic arrest deficient, yeast, homolog)-like 1

Background

It is well known that the nature and relative abundance of individual subunits of different classes of transcription factors can positively or negatively regulate levels of gene expression (1). MAD1 is a transcriptional repressor that competes with Myc for binding to form a sequence specific DNA binding complex (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MAD1L1/MAD1 Antibody (NB500-136)(1)

Publication using NB500-136

• Razeghi, P, Buksinska-Lisik, M, Palanichamy, N, Stepkowski, S, Frazier, O H, Taegtmeyer, H. Transcriptional regulators of ribosomal biogenesis are increased in the unloaded heart, . 20, 1090-1096. 2006-01-01 [PMID: 16770008]

Bioinformatics

• Gene Symbol: MAD1L1
• Entrez:
  • 8379(Human)
  • 17120(Mouse)