MAD1L1/MAD1 Antibody (9B10) - BSA Free

Western Blot: MAD1L1/MAD1 Antibody (9B10) - BSA Free [NB120-5783] - Whole extract of HeLa cells was separated on SDS-PAGE and probed with Anti-Mad1 antibody, Mouse monoclonal. The antibody was developed using 1:10000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat.Lanes:1. 1:1000 antibody2. 1:2000 antibody3. 1:4000 antibody4. Negative control

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clone: 9B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 2.0 mg/ml

MAD1L1/MAD1 Antibody (9B10) - BSA Free Summary

• Description: Novus Biologicals Mouse MAD1L1/MAD1 Antibody (9B10) - BSA Free (NB120-5783) is a monoclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant full length human MAD1 protein. Uniprot accession no. Q9Y6D9
• Localization: Nuclear
• Specificity: MAD1 (9B10)
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAD1L1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10-20 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Western Blot
• Theoretical MW: ~85 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 2.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for MAD1L1/MAD1 Antibody (9B10) - BSA Free

• hMAD1
• HsMAD1
• MAD1 mitotic arrest deficient-like 1 (yeast)
• MAD1
• MAD1L1
• MAD1-like protein 1
• MAD1yeast, homolog-like 1
• Mitotic checkpoint MAD1 protein homolog
• PIG9
• Tax-binding protein 181
• TP53I9
• tumor protein p53 inducible protein 9
• TXBP181
• TXBP181MAD1 (mitotic arrest deficient, yeast, homolog)-like 1

Background

Mitotic checkpoint prevents the transition of eukaryotic cells to anaphase until all the chromosomes have made productive, bipolar attachments through their kinetochores to the microtubules of the mitotic spindle. In Saccharomyces cervisiae six genes are important for the kinetochore-dependent mitotic checkpoint: MAD1, MAD2, MAD3, BUB1, BUB3, and Spindles gene MPS1. All six genes have homologues in higher eukaryotes.1-3 In mammals the mitotic checkpoint is an important mechanism that controls the advance to anaphase during every mitosis. Gene inactivation of MAD2 or Bub3 causes lethality in mice and acquisition of aneuploidy in cell culture. Unattached kinetochores are responsible for generating the checkpoint signal that prevents progression to the anaphase stage. Part of the signal is to inhibit the Cdc20-activated form of a ubiquitin ligase, inhibition of the anaphase promoting complex/cyclosome (APC/C) and inhibition of the ubiquitination of substrates whose destruction is important for the cell to advance to the anaphase stage. Human Mad1 can complex with Mad2 and is preferentially localized to unattached kinetochores during mitosis. Both Mad1 and Mad2 localize to the nuclear pore complex throughout interphase.1-3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAD1L1
• Entrez:
  • 8379(Human)