MAD1L1/MAD1 Antibody (9B10)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IP
Clone
9B10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
2.0 mg/ml

MAD1L1/MAD1 Antibody (9B10) Summary

Immunogen
Recombinant full length human MAD1 protein.
Localization
Nuclear
Specificity
MAD1 (9B10)
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MAD1L1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 10-20 ug/ml
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
2.0 mg/ml
Purity
Protein A or G purified

Alternate Names for MAD1L1/MAD1 Antibody (9B10)

  • hMAD1
  • HsMAD1
  • MAD1 mitotic arrest deficient-like 1 (yeast)
  • MAD1
  • MAD1L1
  • MAD1-like protein 1
  • MAD1yeast, homolog-like 1
  • Mitotic checkpoint MAD1 protein homolog
  • PIG9
  • Tax-binding protein 181
  • TP53I9
  • tumor protein p53 inducible protein 9
  • TXBP181
  • TXBP181MAD1 (mitotic arrest deficient, yeast, homolog)-like 1

Background

Mitotic checkpoint prevents the transition of eukaryotic cells to anaphase until all the chromosomes have made productive, bipolar attachments through their kinetochores to the microtubules of the mitotic spindle. In Saccharomyces cervisiae six genes are important for the kinetochore-dependent mitotic checkpoint: MAD1, MAD2, MAD3, BUB1, BUB3, and Spindles gene MPS1. All six genes have homologues in higher eukaryotes.1-3 In mammals the mitotic checkpoint is an important mechanism that controls the advance to anaphase during every mitosis. Gene inactivation of MAD2 or Bub3 causes lethality in mice and acquisition of aneuploidy in cell culture. Unattached kinetochores are responsible for generating the checkpoint signal that prevents progression to the anaphase stage. Part of the signal is to inhibit the Cdc20-activated form of a ubiquitin ligase, inhibition of the anaphase promoting complex/cyclosome (APC/C) and inhibition of the ubiquitination of substrates whose destruction is important for the cell to advance to the anaphase stage. Human Mad1 can complex with Mad2 and is preferentially localized to unattached kinetochores during mitosis. Both Mad1 and Mad2 localize to the nuclear pore complex throughout interphase.1-3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for MAD1L1/MAD1 Antibody (NB120-5783) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB120-5783AF405
Alexa Fluor 488 Labeled NB120-5783AF488
Alexa Fluor 647 Labeled NB120-5783AF647
Alexa Fluor 700 Labeled NB120-5783AF700
DyLight 350 Labeled NB120-5783UV
DyLight 405 Labeled NB120-5783V
DyLight 488 Labeled NB120-5783G
DyLight 680 Labeled NB120-5783FR
DyLight 755 Labeled NB120-5783IR

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Bioinformatics Tool for MAD1L1/MAD1 Antibody (NB120-5783)

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Bioinformatics

Gene Symbol MAD1L1
Entrez