Human Macrophage Inflammatory Protein 1 alpha ELISA Kit (Colorimetric)

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ELISA: Human Macrophage Inflammatory Protein 1 alpha ELISA Kit (Colorimetric) [NBP3-39771] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids
Standard Curve Range
15.625 - 1000 pg/mL (example only; lot dependent)
Sensitivity
5.9 pg/mL (example only; lot dependent)

Order Details

Human Macrophage Inflammatory Protein 1 alpha ELISA Kit (Colorimetric) Summary

Description
Assay Length: 4.5 hours
Standard Curve Range
15.625 - 1000 pg/mL (example only; lot dependent)
Sensitivity
5.9 pg/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CCL3

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Reagent A
  2. Detection Reagent B
  3. Diluent Buffer
  4. Instruction manual
  5. Plate sealer for 96 wells
  6. Pre-coated 96T strip plate
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human Macrophage Inflammatory Protein 1 alpha ELISA Kit (Colorimetric)

  • C-C motif chemokine 3
  • chemokine (C-C motif) ligand 3
  • G0S19-1Tonsillar lymphocyte LD78 alpha protein
  • LD78ALPHA
  • Macrophage inflammatory protein 1-alpha
  • MIP1AG0/G1 switch regulatory protein 19-1
  • MIP-1-alphaSIS-beta
  • SCYA3PAT 464.1
  • small inducible cytokine A3 (homologous to mouse Mip-1a)
  • Small-inducible cytokine A3

Background

Macrophage Inflammatory Protein 1 alpha (MIP1 alpha) and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor-1 (C-C CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CCL3