Chicken Lysozyme ELISA Kit (Colorimetric)

ELISA: Chicken Lysozyme ELISA Kit (Colorimetric) [NBP2-60088] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Chicken Lysozyme ELISA Kit (Colorimetric) [NBP2-60088] - Standard Curve Reference

• Reactivity: Ch
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and egg white
• Standard Curve Range: 15.625 ng/mL - 4000 ng/mL
• Sensitivity: 14.876 ng/mL

Chicken Lysozyme ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of chicken lysozyme. No significant cross-reactivity or interference between chicken lysozyme and analogues was observed.
• Standard Curve Range: 15.625 ng/mL - 4000 ng/mL
• Sensitivity: 14.876 ng/mL
• Assay Type: Competitive ELISA
• Inter-Assay: %CV < 10
• Intra-Assay: %CV < 8
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: LYZ

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Chicken Lysozyme ELISA Kit (Colorimetric)

• EC 3.2.1.17
• lysozyme (renal amyloidosis)1,4-beta-N-acetylmuramidase C
• lysozyme C
• Lysozyme
• LZM
• renal amyloidosis

Background

This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for lysozyme has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated lysozyme. A competitive inhibition reaction is launched between lysozyme (Standards or samples) and biotin-conjugated lysozyme with the pre-coated lysozyme antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of lysozyme bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: LYZ