Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 532

Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human KDM4C
  Lys381-Lys588
  Accession # Q9H3R0
• Specificity: Detects human KDM4C in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human KDM4A is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [Alexa Fluor® 532]

• EC 1.14.11
• EC 1.14.11.-
• GASC-1 protein
• GASC1
• GASC1JmjC domain-containing histone demethylation protein 3C
• Gene amplified in squamous cell carcinoma 1 protein
• JHDM3C
• JMJD2C
• JMJD2CbA146B14.1
• jumonji domain containing 2C
• Jumonji domain-containing protein 2C
• KDM4C
• KIAA0780FLJ25949
• Lysine (K)specific Demethylase 4C
• Lysine (K)-specific Demethylase 4C
• lysine-specific demethylase 4C

Background

KDM4C (Lysine [K]-specific demethylase 4C; also GASC1 and JMJD2C) is a 120-140 kDa member of the JHDM3 histone demethylase family of enzymes. It is ubiquitously expressed, and specifically demethylates trimethylated lysines on histone H3 (on Lys9 and Lys36), as well as multiple non-histone methylated proteins such as WIZ, CDYL1 and G9a. Transcriptional activation occurs when it interacts with the androgen and progesterone receptors. Human KDM4C is 1056 amino acids (aa) in length. It contains one jumonji N domain (aa 16-58), a catalytic jumonji C domain (aa 144-310), two PHD zinc finger regions (aa 689-865), and two TUDOR methylated-histone binding domains (aa 877-991). Three potential splice variants are reported. One demonstrates an alternative start site 23 aa upstream of the standard site, a second shows a 49 aa substitution for aa 999-1056, and a third contains a five aa substitution for aa 809-1056. Over aa 381-588, human KDM4C shares 51% aa identity with mouse KDM4C.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.