Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Knockout HeLa Cell Lysate

Product Discontinued

Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,1 bp insertion in exon 3
• Gene: KDM4A

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Knockout HeLa Cell Lysate

• EC 1.14.11
• EC 1.14.11.-
• JHDM3A
• JHDM3AJMJD2
• JmjC domain-containing histone demethylation protein 3A
• JMJD2
• JMJD2A
• JMJD2Alysine-specific demethylase 4A
• jumonji domain containing 2
• jumonji domain containing 2A
• Jumonji domain-containing protein 2A
• KDM4A
• KIAA0677jumonji C domain-containing histone demethylase 3A
• Lysine (K)specific Demethylase 4A
• Lysine (K)-specific Demethylase 4A

Background

This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq, Apr 2009]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: KDM4A