LYRIC Antibody (2F11C3)

Images

 
Western Blot: LYRIC Antibody (2F11C3) [NBP1-51585] - Western blot analysis using Metadherin mouse mAb against K562 (1), SKBR-3 (2), T47D (3), Hela (4) and MCF-7 (5) cell lysate.
Immunohistochemistry-Paraffin: LYRIC Antibody (2F11C3) [NBP1-51585] - Immunohistochemical analysis of paraffin-embedded human Liver tissues using Metadherin mouse mAb
Flow Cytometry: LYRIC Antibody (2F11C3) [NBP1-51585] - Flow cytometric analysis of Hela cells using Metadherin mouse mAb (green) and negative control (purple).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P
Clone
2F11C3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

LYRIC Antibody (2F11C3) Summary

Immunogen
Purified recombinant fragment of human Metadherin expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MTDH
Purity
Ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-51585 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
50% glycerol
Purity
Ascites

Alternate Names for LYRIC Antibody (2F11C3)

  • 3D3
  • AEG1
  • AEG-13D3/LYRIC
  • AEG1LYRIC/3D3
  • Astrocyte elevated gene-1 protein
  • LYRIC
  • LYRICMetastasis adhesion protein
  • Lysine-rich CEACAM1 co-isolated protein
  • Metadherin
  • MTDH
  • protein LYRIC

Background

LYRIC is a type II membrane protein, the precise role of which has yet to be defined. It is expressed in breast and prostate carcinoma and is a potential mediator of lung metastasis. It has also been found in mouse Purkinje neurons and cerebellum. In epithelial cells it is recruited to tight junctions (TJ) during the maturation of the TJ complexes. LYRIC co-localizes with beta-catenin in normal and tumor tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LYRIC Antibody (NBP1-51585)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(2)
All Applications
Filter By Species
Human
(2)
All Species

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for LYRIC Antibody (NBP1-51585). (Showing 1 - 2 of 2 FAQs).

  1. Could you give me some details about the NBP1-51585? Especially the epitope, I really want to know it exactly.
    • The exact immunogen sequence is proprietary, but the immunogen range is between 378-579. The antibody has not been epitope mapped to our knowledge.
  2. I was just wondering whether the HeLa cells used in the flow cytometry were permeabilised or not? As I am interested in looking whether LYRIC is surface expressed on my cell type.
    • The HeLa cells were permeabilised by using 0.1% Triton 100.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MTDH
Entrez