| Description | Recombinant bioactive protein containing 171 amino acids for Human Lymphotoxin-alpha/TNF-beta Source:E. coli |
| Preparation Method |
Determined to be >98% pure by SDS-PAGE |
| Details of Functionality | The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is < 0.05 ng/ml, corresponding to a specific activity of >2 x 107 units/mg. |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Gene | LTA |
| Purity | >98%, by SDS-PAGE |
| Endotoxin Note | <0.1 ng/ug |
| Dilutions |
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| Theoretical MW | 18.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | Sterile filtered and lyophilized with no additives |
| Concentration | Lyoph |
| Purity | >98%, by SDS-PAGE |
| Reconstitution Instructions | Reconstitute in PBS to a concentration of 1 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for long-term storage. For long term storage, it is recommended to add a carrier protein (0.1% HAS or BSA). |
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| Gene Symbol | LTA |