Recombinant Human Lymphotoxin-alpha/TNF-beta Protein (NBP1-41147)

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SDS-Page: TNF beta Protein [NBP1-41147] - TNF-beta/LTA, 20.9 kDa (192aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human Lymphotoxin-alpha/TNF-beta Protein Summary

Description
A recombinant protein with a His tag and corresponding to amino acids 35 - 205 of LTA.

Source: E. coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL

Preparation
Method
E.coli
Details of Functionality
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is less or equal to 1 ng/ml.
Protein/Peptide Type
Recombinant Protein
Gene
LTA
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Application Notes
Assay
L929 cytotoxicity assay using actinomycin D
Cell Number: 2 x 10,000 cells/well
Incubation: 24hr after sample treatment
Assay Media: 5% FBS media
Cytokine Concentration: 12 pg/ml - 25 ng/ml
Detection method: MTT assay
Theoretical MW
20.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human protein.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 30% glycerol, 0.1M NaCl, 1mM DTT
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Notes

The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 20.9 kDa (192aa), confirmed by MALDI-TOF.

Alternate Names for Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

  • LT
  • LTA
  • LT-alpha
  • lymphotoxin alpha (TNF superfamily, member 1)
  • Lymphotoxin alpha
  • Lymphotoxinalpha
  • Lymphotoxin-alpha
  • tnfb
  • TNF-beta
  • TNFBlymphotoxin-alpha
  • TNFSF1
  • TNFSF1B
  • TNFSF1TNF-beta
  • tumor necrosis factor beta
  • Tumor necrosis factor ligand superfamily member 1

Background

TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Recombinant TNF beta protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol LTA
Entrez

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