Human Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric)

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ELISA: Human Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric) [NBP1-83730] - Representative standard curve for human TNF beta ELISA. Human TNF beta was diluted in serial 2-fold steps inSample Diluent. Do not use this ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma
Standard Curve Range
15.6 - 1,000 pg/mLml
Sensitivity
4.6 pg/mL

Order Details

Human Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric) Summary

Specificity
The assay detects recombinant, unglycosylated lymphotoxin with the same sensitivity as the natural, glycosylated protein, shows good correlation with the standard cytotoxicity bioassay, and is specific for biologically active TNF beta without TNF alpha cross reactivity.
Standard Curve Range
15.6 - 1,000 pg/mLml
Sensitivity
4.6 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
10.2%
Intra-Assay
8.0%
Spike Recovery
The recovery ranged from 87% to 130% with an overall mean recovery of 105%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
LTA

Applications/Dilutions

Application Notes
The human Lymphotoxin-alpha/TNF-beta ELISA is an enzyme-linked immunosorbent assay for quantitative detection.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Stop Solution (1M Phosphoric acid)
  2. Sample Diluent
  3. Substrate Solution (tetramethyl-benzidine)
  4. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  5. Human Caspase-8 Standard lyophilized, 20.00 ng/ml upon reconstitution
  6. Lysis Buffer 10x
  7. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  8. Anti-rabbit-IgG-HRP
  9. anti-human Caspase-8 polyclonal (rabbit) Detection Antibody
  10. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Caspase-8
  11. Adhesive Films

Alternate Names for Human Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric)

  • LT
  • LTA
  • LT-alpha
  • lymphotoxin alpha (TNF superfamily, member 1)
  • Lymphotoxin alpha
  • Lymphotoxinalpha
  • Lymphotoxin-alpha
  • tnfb
  • TNF-beta
  • TNFBlymphotoxin-alpha
  • TNFSF1
  • TNFSF1B
  • TNFSF1TNF-beta
  • tumor necrosis factor beta
  • Tumor necrosis factor ligand superfamily member 1

Background

Tumor Necrosis Factor (TNF) was originally discovered as a serum protein with necrotizing effects on certain transplantable mouse tumors in vivo and cytotoxic effects against some transformed cells in vitro. The TNF family consists of two proteins designated TNF-alpha, also called cachectin, and TNF-beta, also called lymphotoxin. Soluble TNF-beta is a T-cell derived glycoprotein of 25 kD mainly produced by activated lymphocytes. TNF-beta is 28 % structurally homologous with the macrophage produced non-glycoprotein TNF-alpha.TNF-beta is induced in an antigen-specific MHC restricted fashion from class I and class II restricted T cells. Viral infection is also associated with TNF-beta production by lymphoid cells. TNF-beta has several effects on target cells including killing, growth stimulation, induction of adhesion molecule (ICAM-1) expression, and induction of differentiation. The mechanisms of TNF-beta effects involve receptor binding and internalization and several sequelae including changes in prostaglandins and chromosome integrity. TNF-beta participates in tumor immunity, and it has been reported to inhibit carcinogenesis as well as growth of some tumors in vivo.The assay detects recombinant, unglycosylated lymphotoxin with the same sensitivity as the natural, glycosylated protein, shows good correlation with the standard cytotoxicity bioassay, and is specific for biologically active TNF-beta without TNF-alpha cross reactivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol LTA
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