Human Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric)

ELISA: Human Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric) [NBP1-83730] - Representative standard curve for human TNF beta ELISA. Human TNF beta was diluted in serial 2-fold steps inSample Diluent. Do not use this standard curve to derive test results. A standard curve must be run for each group ofmicrowell strips assayed.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma, urine, synovial fluid, amniotic fluid
• Standard Curve Range: 15.6 - 1,000 pg/mLml
• Sensitivity: 4.6 pg/mL

Human Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric) Summary

• Description: The human Lymphotoxin-alpha/TNF-beta ELISA is an enzyme-linked immunosorbent assay for quantitative detection.
• Specificity: The assay detects recombinant, unglycosylated lymphotoxin with the same sensitivity as the natural, glycosylated protein, shows good correlation with the standard cytotoxicity bioassay, and is specific for biologically active TNF beta without TNF alpha cross reactivity.
• Standard Curve Range: 15.6 - 1,000 pg/mLml
• Sensitivity: 4.6 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10.2%
• Intra-Assay: CV% < 8.0%
• Spike Recovery: The recovery ranged from 87% to 130% with an overall mean recovery of 105%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: LTA

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric)

• LT
• LTA
• LT-alpha
• lymphotoxin alpha (TNF superfamily, member 1)
• Lymphotoxin alpha
• Lymphotoxinalpha
• Lymphotoxin-alpha
• tnfb
• TNF-beta
• TNFBlymphotoxin-alpha
• TNFSF1
• TNFSF1B
• TNFSF1TNF-beta
• tumor necrosis factor beta
• Tumor necrosis factor ligand superfamily member 1

Background

Tumor Necrosis Factor (TNF) was originally discovered as a serum protein with necrotizing effects on certain transplantable mouse tumors in vivo and cytotoxic effects against some transformed cells in vitro. The TNF family consists of two proteins designated TNF-alpha, also called cachectin, and TNF-beta, also called lymphotoxin. Soluble TNF-beta is a T-cell derived glycoprotein of 25 kD mainly produced by activated lymphocytes. TNF-beta is 28 % structurally homologous with the macrophage produced non-glycoprotein TNF-alpha.TNF-beta is induced in an antigen-specific MHC restricted fashion from class I and class II restricted T cells. Viral infection is also associated with TNF-beta production by lymphoid cells. TNF-beta has several effects on target cells including killing, growth stimulation, induction of adhesion molecule (ICAM-1) expression, and induction of differentiation. The mechanisms of TNF-beta effects involve receptor binding and internalization and several sequelae including changes in prostaglandins and chromosome integrity. TNF-beta participates in tumor immunity, and it has been reported to inhibit carcinogenesis as well as growth of some tumors in vivo.The assay detects recombinant, unglycosylated lymphotoxin with the same sensitivity as the natural, glycosylated protein, shows good correlation with the standard cytotoxicity bioassay, and is specific for biologically active TNF-beta without TNF-alpha cross reactivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Lymphotoxin-alpha/TNF-beta ELISA Kit (NBP1-83730)(1)

Publication using NBP1-83730

• Takeshita A, Nishida K, Yoshida A Et Al. RANKL expression in chondrocytes and its promotion by lymphotoxin-alpha in the course of cartilage destruction during rheumatoid arthritis PloS one 2021-07-07 [PMID: 34234380] (ELISA)

FAQs for Lymphotoxin-alpha/TNF-beta ELISA Kit (NBP1-83730). (Showing 1 - 2 of 2 FAQ).

1.  Can you tell me whether any preservatives are contained in HRP-Conjugate, Standard and Sample Diluent?
   •  The NBP1-83730 does not contain any preservatives in the conjugate, standard and sample diluent and there is nothing hazardous in any of the reagents. 
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: LTA