LYK5 Antibody

Western Blot: LYK5 Antibody [NBP2-17187] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000

Immunocytochemistry/Immunofluorescence: LYK5 Antibody [NBP2-17187] - Analysis of MCF-7 cells. Green: LYK5 protein stained by LYK5 antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: LYK5 Antibody [NBP2-17187] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.

Western Blot: LYK5 Antibody [NBP2-17187] - Sample (30 ug of whole cell lysate) A: 293T B: HepG2 12% SDS PAGE diluted at 1:1000

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

LYK5 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human LYK5. The exact sequence is proprietary.
• Localization: Nucleus; Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STRADA
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for LYK5 Antibody

• LYK5FLJ90524
• NY-BR-96
• protein kinase LYK5
• Serologically defined breast cancer antigen NY-BR-96
• STE20-like pseudokinase
• STE20-related adapter protein
• STE20-related adaptor protein
• STE20-related kinase adapter protein alpha
• STE20-related kinase adaptor alpha
• Stlk
• STRAD alpha
• STRADPMSE

Background

The protein encoded by this gene contains a STE20-like kinase domain, but lacks several residues that are critical for catalytic activity, so it is termed a 'pseudokinase'. The protein forms a heterotrimeric complex with serine/threonine kinase 11 (STK11, also known as LKB1) and the scaffolding protein calcium binding protein 39 (CAB39, also known as MO25). The protein activates STK11 leading to the phosphorylation of both proteins and excluding STK11 from the nucleus. The protein is necessary for STK11-induced G1 cell cycle arrest. A mutation in this gene has been shown to result in polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE) syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their full-length nature is not known. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: STRADA