Ly-6C Antibody (HK1.4) - Azide and BSA Free

Flow Cytometry: Ly-6C Antibody (HK1.4) [NBP1-28046] - BALB/c mouse bone marrow cells were stained with Rat Anti-Mouse Ly-6C-UNLB followed by Mouse Anti-Rat IgG2c-FITC.

Flow Cytometry: Ly-6C Antibody (HK1.4) [NBP1-28046] - 1 ug/10^6 bone marrow cells from C57BL/6 mice were labeled with purified rat anti-mouse Ly-6C and then stained with goat anti-rat IgG-FITC. Large cells were gated and analyzed by flow cytometry.

Flow Cytometry: Ly-6C Antibody (HK1.4) [NBP1-28046] - Analysis using the Biotin conjugate of NBP1-28046. Staining of BALB/c bone marrow cells.

Flow Cytometry: Ly-6C Antibody (HK1.4) [NBP1-28046] - Analysis using the FITC conjugate of NBP1-28046. Staining of 1 ug on Balb/c Bone Marrow.

• Reactivity: Mu
• Applications: Flow
• Clone: HK1.4
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

Ly-6C Antibody (HK1.4) - Azide and BSA Free Summary

• Immunogen: L3 cloned CTL cells
• Specificity: Murine Ly-6C, Mr 18 kDa
• Isotype: IgG2c Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: Ly6c1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry <= 1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 1.0mM BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Ly-6C Antibody (HK1.4) - Azide and BSA Free

• Ly 6c
• Ly6c protein
• Lymphocyte antigen 6 complex locus C
• Lymphocyte antigen 6 complex
• Lymphocyte antigen 6C
• Lymphocyte antigen Ly 6C

Background

Ly-6C is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T-cell subsets. Mice with the Ly-6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly-6C+ and CD8+Ly-6C+ cells, while Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly-6C+ lymphocytes. Ly-6C may play a role in the development and maturation of lymphocytes. (1-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ly-6C Antibody (NBP1-28046)(4)

Publications using NBP1-28046

• Fortin J, Chiang MF, Meydan C et Al. Distinct and opposite effects of leukemogenic Idh and Tet2 mutations in hematopoietic stem and progenitor cells Proc Natl Acad Sci U S A 2023-01-18 [PMID: 36652477]
• Salman S, Meyers DJ, Wicks EE Et al. HIF inhibitor 32-134D eradicates murine hepatocellular carcinoma in combination with anti-PD1 therapy J Clin Invest 2022-05-02 [PMID: 35499076] (FLOW, Mouse)
  
  Details:
  Citation using the Alexa Fluor 488 version of this antibody.
• Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (FLOW, Mouse)
  
  Details:
  Citation using the PE format of this antibody.
• Novince CM, Whittow CR, Aartun JD et al. Commensal Gut Microbiota Immunomodulatory Actions in Bone Marrow and Liver have Catabolic Effects on Skeletal Homeostasis in Health Sci Rep 2017-07-18 [PMID: 28720797] (FLOW, Mouse)

Bioinformatics

• Gene Symbol: Ly6c1