Lubricin/PRG4 Antibody

Western Blot: Proteoglycan 4 Antibody [NB100-74453] - Analysis of 3ug of non-reduced human synovial fluid per well onto a SDS-PAGE gel.

Western Blot: Proteoglycan 4 Antibody [NB100-74453] - Analysis of Human recombinant Lubricin.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Lubricin/PRG4 Antibody Summary

• Description: Novus Biologicals Rabbit Lubricin/PRG4 Antibody (NB100-74453) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to residues C L(1356) P N I R K Q P D G Y D Y Y A F S K D Q(1374) of human Lubricin.
• Localization: Secreted
• Specificity: Detects Lubricin in human samples.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRG4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot 2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Lubricin/PRG4 Antibody

• bG174L6.2 (MSF: megakaryocyte stimulating factor )
• bG174L6.2
• CACP
• FLJ32635
• HAPO
• Hemangiopoietin
• JCAP
• Lubricin
• megakaryocyte stimulating factor
• Megakaryocyte-stimulating factor
• MSF
• MSFproteoglycan 4, (megakaryocyte stimulating factor, articular superficial zoneprotein, camptodactyly, arthropathy, coxa vara, pericarditis syndrome)
• PRG4
• Proteoglycan 4
• Superficial zone proteoglycan
• SZP
• SZPHAPOarticular superficial zone protein

Background

Lubricin is a glycoprotein encoded by the PRG4 gene. It has been identified as a component of joint lubrication and synovial homeostasis by reducing friction in joints and preventing synovial cell overgrowth. It is a large, water soluble molecule that acts as a carrier for insoluble surface-active phospholipid (SAPL). Depletion of lubricin function has been associated with camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), an arthritic-like autosomal recessive disorder.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRG4
• Entrez:
  • 10216(Human)