LSM1 Antibody - Azide and BSA Free

Immunoprecipitation: LSM1 Antibody [NBP3-00249] - LSM1 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 2 uL NBP3-00249 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00249, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 15 kDa Observed band size: 15 kDa

Immunohistochemistry-Paraffin: LSM1 Antibody [NBP3-00249] - Immunochemical staining of human LSM1 in human prostatic carcinoma with NBP3-00249 (1:10000, formalin-fixed paraffin embedded sections).

Immunohistochemistry-Paraffin: LSM1 Antibody [NBP3-00249] - Immunochemical staining of human LSM1 in human lung carcinoma with NBP3-00249 (1:10000, formalin-fixed paraffin embedded sections).

Western Blot: LSM1 Antibody [NBP3-00249] - NBP3-00249 at 1:500 dilution Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:15 kDa Observed band size:15 kDa

Immunohistochemistry-Paraffin: LSM1 Antibody [NBP3-00249] - Immunochemical staining of human LSM1 in human breast carcinoma with NBP3-00249 (1:10000, formalin-fixed paraffin embedded sections).

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

LSM1 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human LSM1 (Accession#: O15116; Met1-Tyr133)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LSM1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunohistochemistry-Paraffin 1:2500-1:10000
  • Immunoprecipitation 0.5-2 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for LSM1 Antibody - Azide and BSA Free

• Cancer-associated Sm-like
• CASMYJL124C
• LSM1 homolog, U6 small nuclear RNA associated (S. cerevisiae)
• Small nuclear ribonuclear CaSm
• U6 snRNA-associated Sm-like protein LSm1

Background

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; MIM 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LSM1