LRRC4 Antibody

LRRC4 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 15 µg/mL Sheep Anti-Human LRRC4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4995) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

LRRC4 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human LRRC4
  Ala39-Lys527
  Accession # Q9HBW1
• Specificity: Detects human LRRC4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human LRRC3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: LRRC4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRRC4 Antibody

• BAG
• Brain Tumor Associated Protein LRRC4
• Brain Tumor-Associated Protein BAG
• Leucine Rich Repeat Containing 4
• Leucine-Rich Repeat-Containing 4
• Leucine-Rich Repeat-Containing Protein 4
• LRRC4
• NAG14
• Nasopharyngeal Carcinoma-Associated Gene 14 Protein
• Netrin-G2 Ligand
• NGL-2

Background

LRRC4 (Leu-rich repeat/LRR-containing protein 4; also brain tumor-associated protein) is a 55 kDa (predicted) member of the Leu-rich protein family. It is expressed on neurons and astrocytes, and antagonizes the signaling pathways for cytokines and growth factors. It is reduced in expression in tumors. Human LRRC4 is a type I transmembrane protein that is 653 amino acids (aa) in length. It contains a 489 aa extracellular region (aa 39-527), and a 105 aa cytoplasmic domain. The extracellular region shows nine LRRs (aa 74-288), a C2 type Ig-like domain (aa 354-440), and a Thr rich segment (aa 455-526). Over aa 39-527, human LRRC4 is 98% aa identical to both mouse and canine LRRC4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LRRC4 Antibody (AF4995)(1)

Publication using AF4995

• Peer-Hendrik Kuhn, Alessio Vittorio Colombo, Benjamin Schusser, Daniela Dreymueller, Sebastian Wetzel, Ute Schepers et al. Systematic substrate identification indicates a central role for the metalloprotease ADAM10 in axon targeting and synapse function eLife 2016-01-23 [PMID: 26802628]

Bioinformatics

• Gene Symbol: LRRC4
• Uniprot:
  • Q9HBW1()