LRRC32/GARP Antibodies

HAF016). A specific band was detected for LRRC32/GARP at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for LRRC32/GARP at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse spleen non-B cells. PVDF Membrane was probed with 0.1 µg/mL of Sheep Anti-Mouse LRRC32/GARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6229) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRRC32/GARP at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VCTS021). Tissue was stained using the Donkey Anti-Sheep IgG HRP Affinity Purified PAb (brown; Catalog # HAF016) and counterstained with hematoxylin (blue). Specific staining was localized to extracellular space. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VCTS021). Tissue was stained using the Donkey Anti-Sheep IgG HRP Affinity Purified PAb (brown; Catalog # HAF016) and counterstained with hematoxylin (blue). Specific staining was localized to extracellular space. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

240-B), and 20 ng/mLRecombinant Human IL-2 (Catalog # 202-IL) for 48 hours were stained with Mouse Anti-Human/Mouse LAP TGF-beta 1 APC-conjugated Monoclonal Antibody (Catalog # FAB2463A) and either (A) Mouse Anti-Human LRRC32/GARP Monoclonal Antibody (Catalog # MAB6055) or (B) Rat IgG₁Isotype Control (Catalog # MAB005) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." class="big_lightbox"> 240-B), and 20 ng/mLRecombinant Human IL-2 (Catalog # 202-IL) for 48 hours were stained with Mouse Anti-Human/Mouse LAP TGF-beta 1 APC-conjugated Monoclonal Antibody (Catalog # FAB2463A) and either (A) Mouse Anti-Human LRRC32/GARP Monoclonal Antibody (Catalog # MAB6055) or (B) Rat IgG₁Isotype Control (Catalog # MAB005) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." alt="Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Regulatory T Cells (Tregs) with 10 µg/mL Anti-CD3, 5 µg/mL Anti-CD28, 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B), and 20 ng/mLRecombinant Human IL-2 (Catalog # 202-IL) for 48 hours were stained with Mouse Anti-Human/Mouse LAP TGF-beta 1 APC-conjugated Monoclonal Antibody (Catalog # FAB2463A) and either (A) Mouse Anti-Human LRRC32/GARP Monoclonal Antibody (Catalog # MAB6055) or (B) Rat IgG₁Isotype Control (Catalog # MAB005) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." class="big_thumb" />

402-ML), and Human TGF-beta 1 (Catalog # 100-B) for 3 days to induce T regulatory cells (Tregs). Tregs were then stained with Rat Anti-Mouse CD4 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB554G), Phycoerythrin-conjugated Anti-FoxP3, and either (A) Rat Anti-Mouse LRRC32/GARP Monoclonal Antibody (Catalog # MAB62291) or (B) Rat IgG1 Isotype Control (Catalog # MAB005) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Dot plots are gated on CD4+ T cells." class="big_lightbox"> 402-ML), and Human TGF-beta 1 (Catalog # 100-B) for 3 days to induce T regulatory cells (Tregs). Tregs were then stained with Rat Anti-Mouse CD4 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB554G), Phycoerythrin-conjugated Anti-FoxP3, and either (A) Rat Anti-Mouse LRRC32/GARP Monoclonal Antibody (Catalog # MAB62291) or (B) Rat IgG1 Isotype Control (Catalog # MAB005) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Dot plots are gated on CD4+ T cells." alt="Mouse splenocytes were stimulated with 10 µL/mL anti-CD3/anti-CD28, Recombinant Mouse IL-2 (Catalog # 402-ML), and Human TGF-beta 1 (Catalog # 100-B) for 3 days to induce T regulatory cells (Tregs). Tregs were then stained with Rat Anti-Mouse CD4 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB554G), Phycoerythrin-conjugated Anti-FoxP3, and either (A) Rat Anti-Mouse LRRC32/GARP Monoclonal Antibody (Catalog # MAB62291) or (B) Rat IgG1 Isotype Control (Catalog # MAB005) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Dot plots are gated on CD4+ T cells." class="big_thumb" />