LRP2 Antibody (545606) [Alexa Fluor® 647]

HEK293 cells transfected with Human LRP2 were stained with Mouse Anti-Human Megalin/LRP2 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB9578R, filled histogram) or irrelevant HEK293 cells (open histogram). To facilitate intracellular staining, cells were fixed with FC004 and permeabilized with Saponin . View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 545606
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

LRP2 Antibody (545606) [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Megalin/LRP2
  Pro3510-Lys3964
  Accession # P98164
• Specificity: Detects human Megalin/LRP2 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRP2 Antibody (545606) [Alexa Fluor® 647]

• calcium sensor protein
• DBS
• DBSlow-density lipoprotein receptor-related protein 2
• EC 1.1.2.3
• EC 3.4.21.9
• Glycoprotein 330
• gp330
• Heymann nephritis antigen homolog
• low density lipoprotein receptor-related protein 2
• LRP2
• LRP-2
• Megalin

Background

Megalin, also known as the low-density lipoprotein receptor-related protein 2 (LRP2), is a large type I transmembrane cell surface protein. This glycoprotein is a multi‑ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney (1). The Megalin protein is critical for the re-uptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling. Mutations in this gene cause Donnai-Barrow Syndrome (DBS) and Facio-Oculoacoustico-Renal Syndrome (FOAR) (1). Megalin is consisting of a 25 amino acid (aa) probable N‑terminal signal peptide sequence, a 4400 aa extracellular region, a 22 aa single transmembrane domain, and a 213 aa C‑terminal cytoplasmic tail. The entire extracellular region is made up of 36 class A motifs of putative ligand-binding domains arranged in four distinct clusters, 16 growth factor repeats separated by 8 YWTD spacer regions, and 1 epidermal growth factor-like repeat (2). The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase (3). The amino acid 3510-3964 encodes the fourth class A motif cluster in human Megalin, termed Megalin C4. Human Megalin C4 shares 77% and 74% identity with mouse and rat Megalin C4.

1. Christensen, E. I. and Birn, H. (2002) Nat. Rev. Mol. Cell Biol 3:256.
2. Saito, A. et al. (1994) Proc.Natl. Aca. Sci. U. S. A. 91:9725.
3. Kantarci, S. et al. (2007) Nat. Genet 39:957.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LRP2 Antibody (FAB9578R)(1)

Publication using FAB9578R

• Glover, EK;Berlinguer-Palmini, R;Thompson, ER;Wilson, C;Denby, L;Reynolds, G;Ali, S;Lowe, M;Lennon, R;Sheerin, NS; AntimiR uptake by human proximal tubule epithelial cells is predominantly by macropinocytosis Scientific reports 2025-08-29 [PMID: 40877447] (Flow Cytometry, Human)

Bioinformatics

• Uniprot:
  • P98164()