LRP2 Antibody (545606) [Alexa Fluor™ Plus 594]

• Reactivity: Hu
• Clone: 545606
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 594

LRP2 Antibody (545606) [Alexa Fluor™ Plus 594] Summary

• Specificity: Detects human Megalin/LRP2 in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRP2 Antibody (545606) [Alexa Fluor™ Plus 594]

• calcium sensor protein
• DBS
• DBSlow-density lipoprotein receptor-related protein 2
• EC 1.1.2.3
• EC 3.4.21.9
• Glycoprotein 330
• gp330
• Heymann nephritis antigen homolog
• low density lipoprotein receptor-related protein 2
• LRP2
• LRP-2
• Megalin

Background

Megalin, also known as the low-density lipoprotein receptor-related protein 2 (LRP2), is a large type I transmembrane cell surface protein. This glycoprotein is a multi‑ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney (1). The Megalin protein is critical for the re-uptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling. Mutations in this gene cause Donnai-Barrow Syndrome (DBS) and Facio-Oculoacoustico-Renal Syndrome (FOAR) (1). Megalin is consisting of a 25 amino acid (aa) probable N‑terminal signal peptide sequence, a 4400 aa extracellular region, a 22 aa single transmembrane domain, and a 213 aa C‑terminal cytoplasmic tail. The entire extracellular region is made up of 36 class A motifs of putative ligand-binding domains arranged in four distinct clusters, 16 growth factor repeats separated by 8 YWTD spacer regions, and 1 epidermal growth factor-like repeat (2). The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase (3). The amino acid 3510-3964 encodes the fourth class A motif cluster in human Megalin, termed Megalin C4. Human Megalin C4 shares 77% and 74% identity with mouse and rat Megalin C4.

1. Christensen, E. I. and Birn, H. (2002) Nat. Rev. Mol. Cell Biol 3:256.
2. Saito, A. et al. (1994) Proc.Natl. Aca. Sci. U. S. A. 91:9725.
3. Kantarci, S. et al. (2007) Nat. Genet 39:957.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.