LRP-1B Antibody (1034124) [Unconjugated]

Western blot shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line and U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LRP‑1 beta subunit Monoclonal Antibody (Catalog # MAB2368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for LRP‑1 beta subunit at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

LRP‑1 beta subunit was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human LRP‑1 beta subunit Monoclonal Antibody (Catalog # MAB2368) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 1034124
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

LRP-1B Antibody (1034124) [Unconjugated] Summary

• Immunogen:
  E. coli-derived recombinant human LRP-1
  
  Gln4449-Ala4544
  Accession # Q07954
• Specificity: Detects human LRP-1 beta subunit in ELISA and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRP-1B Antibody (1034124) [Unconjugated]

• EC 1.1.1.94
• EC 3.4.21.9
• low density lipoprotein receptor related protein-deleted in tumor
• low density lipoprotein receptor-related protein 1B
• low density lipoprotein-related protein 1B (deleted in tumors)
• Low-density lipoprotein receptor-related protein-deleted in tumor
• LRP1B
• LRP-1B
• LRP-deleted in tumors
• LRPDIT
• LRP-DIT
• LRP-DITlow-density lipoprotein receptor-related protein 1B

Background

LRP-1 (low-density lipoprotein receptor-related protein 1) is a large type I transmembrane protein belonging to the LDL receptor superfamily. The extracellular domain of LRP-1 is organized in four clusters of ligand-binding repeats that recognizes at least 30 different ligands. The amino acid sequence of human and mouse LRP-1 cluster II share 100% identity. LRP-1 is an endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. It plays a crucial role in early embryonic development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q07954(Human)