LRG1 Overexpression Lysate (Native)

Images

 
Western Blot: LRG1 Overexpression Lysate (Adult Normal) [NBL1-12665] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LRG1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

LRG1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC206780 and based on accession number NM_052972. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens leucine-rich alpha-2-glycoprotein 1 (LRG1), mRNA.
Gene
LRG1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for LRG1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for LRG1 Overexpression Lysate (Native)

  • 1300008B03Rik
  • 2310031E04Rik
  • HMFT1766
  • leucine-rich alpha-2-glycoprotein 1
  • leucine-rich alpha-2-glycoprotein
  • LRG1
  • LRGleucine rich alpha 2 glycoprotein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF4409
Species: Mu
Applications: WB
NBP2-42388
Species: Hu
Applications: WB, IHC, IHC-P
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
AF2747
Species: Mu
Applications: WB, IHC, IP, ELISA(Cap), ELISA(Det), ELISA(Sta)
NBP2-25162
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP1-87679
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB500-418
Species: Hu, Po, Rb
Applications: WB, B/N, ELISA, ICC/IF, IHC, IHC-P, RIA
NB100-56503
Species: Hu, Mu, Rt, Po, Ca, Dr, Eq, Ma, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-52557
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, IF
NBP2-52406
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NB100-56074
Species: Hu, Mu, Rt, Ge
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-42526
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA, S-ELISA, ELISA(Cap), ELISA(Det)
NB110-68136
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, Flow-IC
AF-241-NA
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBP1-81858
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-24664
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow

Publications for LRG1 Lysate (NBL1-12665) (0)

There are no publications for LRG1 Lysate (NBL1-12665).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for LRG1 Lysate (NBL1-12665) (0)

There are no reviews for LRG1 Lysate (NBL1-12665). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LRG1 Lysate (NBL1-12665) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional LRG1 Products

Bioinformatics Tool for LRG1 Lysate (NBL1-12665)

Discover related pathways, diseases and genes to LRG1 Lysate (NBL1-12665). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for LRG1 Lysate (NBL1-12665)

Discover more about diseases related to LRG1 Lysate (NBL1-12665).
 

Pathways for LRG1 Lysate (NBL1-12665)

View related products by pathway.

PTMs for LRG1 Lysate (NBL1-12665)

Learn more about PTMs related to LRG1 Lysate (NBL1-12665).
 

Research Areas for LRG1 Lysate (NBL1-12665)

Find related products by research area.

Blogs on LRG1

There are no specific blogs for LRG1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

GM-CSF [Unconjugated]
215-GM-010/CF
LRG1 Antibody
NBP2-24603

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our LRG1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol LRG1
Entrez