LOX propeptide Antibody

Western Blot: LOX propeptide Antibody [NBP1-30012] - Western blot on cytoplasmic (c) and nuclear (n) extracts of NIH 3T3, ras-transformed (RS 485), PMEF and TGM-1 mouse fibroblast cells.

Immunohistochemistry-Paraffin: LOX propeptide Antibody [NBP1-30012] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human skin using LOX propeptide antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic and plasma membrane staining was observed. Staining was performed by Histowiz.

Simple Western: LOX propeptide Antibody [NBP1-30012] - Simple Western lane view shows a specific band for LOX propeptide in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Discontinued

LOX propeptide Antibody Summary

• Immunogen: A synthetic peptide made to an internal region of mouse LOX propeptide (within residues 135-155). [Swiss-Prot P28301]
• Localization: Secreted extracellular space. Cytoplasm and nucleus (Contente, et al).
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LOX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Simple Western 1:200
  • Western Blot 0.35 ug/ml
• Application Notes: This LOX propeptide antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Western blot, where a band is seen approx. 50 kDa.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NIH 3T3 Whole Cell Lysate

Reactivity Notes

Human and mouse. Immunogen has 100% homology with rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LOX propeptide Antibody

• lysyl oxidaseEC 1.4.3.13
• MGC105112
• protein-lysine 6-oxidase

Background

Lysyl oxidase (LOX) is a critical enzyme in extracellular matrix biosynthesis as well as in the maintainance of normal bone phenotype. It is synthesized/secreted as a 50-kD proenzyme 'pro-LOX' which is then processed to 30-kD mature enzyme 'LOX' and 18-kD lysyl oxidase propeptide 'LOX-PP' by extracellular procollagen C-proteinases (BMP1, Tll1, and Tll2). LOX-PP has been proposed to implicate in the maintenance of inactive state of LOX within the secretory pathway and to act as intramolecular chaperones which facilitate the accurate protein folding/targeting to correct destinations. LOX-PP involves tumor suppression by inhibiting RAS-dependent cellular transformation and its expression has also been associated with inhibition of anchorage-independent progression as well as migration and growth suppression xenografts in Her-2/neu-driven breast cancer. LOX-PP also inhibits PI3K/AKT and ERK1/2 MAP kinase pathways, as well as the levels of downstream NF-kB/cyclin D1 signals in certain cancers - breast, pancreatic, lung, prostate and oral cancers. LOX-PP has also been shown to inhibit DNA synthesis in rat's VSMCs cultures, osteoblast proliferation and FGF-2 signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LOX propeptide Antibody (NBP1-30012)(2)

Publications using NBP1-30012

• Di Stefano V, Torsello B, et al. Major Action of Endogenous Lysyl Oxidase in Clear Cell Renal Cell Carcinoma Progression and Collagen Stiffness Revealed by Primary Cell Cultures. Am J Pathol 2016-09-01 [PMID: 27449199] (WB, Human)
• Contente S, Yeh TJ, Friedman RM. Tumor suppressive effect of lysyl oxidase proenzyme. Biochim Biophys Acta 2009-07-01 [PMID: 19410608]

Bioinformatics

• Gene Symbol: LOX
• Entrez:
  • 4015(Human)
  • 16948(Mouse)
• Uniprot:
  • P28300(Human)
  • P28301(Mouse)