Western Blot: LOX propeptide Antibody [NBP1-30012] - Western blot on cytoplasmic (c) and nuclear (n) extracts of NIH 3T3, ras-transformed (RS 485), PMEF and TGM-1 mouse fibroblast cells.
Immunohistochemistry-Paraffin: LOX propeptide Antibody [NBP1-30012] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human skin using LOX propeptide antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). ...read more
Simple Western: LOX propeptide Antibody [NBP1-30012] - Simple Western lane view shows a specific band for LOX propeptide in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using ...read more
This LOX propeptide antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Western blot, where a band is seen approx. 50 kDa.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human and mouse. Immunogen has 100% homology with rat.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.1% Sodium Azide
Immunogen affinity purified
Alternate Names for LOX propeptide Antibody
lysyl oxidaseEC 22.214.171.124
Lysyl oxidase (LOX) is a critical enzyme in extracellular matrix biosynthesis as well as in the maintainance of normal bone phenotype. It is synthesized/secreted as a 50-kD proenzyme 'pro-LOX' which is then processed to 30-kD mature enzyme 'LOX' and 18-kD lysyl oxidase propeptide 'LOX-PP' by extracellular procollagen C-proteinases (BMP1, Tll1, and Tll2). LOX-PP has been proposed to implicate in the maintenance of inactive state of LOX within the secretory pathway and to act as intramolecular chaperones which facilitate the accurate protein folding/targeting to correct destinations. LOX-PP involves tumor suppression by inhibiting RAS-dependent cellular transformation and its expression has also been associated with inhibition of anchorage-independent progression as well as migration and growth suppression xenografts in Her-2/neu-driven breast cancer. LOX-PP also inhibits PI3K/AKT and ERK1/2 MAP kinase pathways, as well as the levels of downstream NF-kB/cyclin D1 signals in certain cancers - breast, pancreatic, lung, prostate and oral cancers. LOX-PP has also been shown to inhibit DNA synthesis in rat's VSMCs cultures, osteoblast proliferation and FGF-2 signaling.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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