LOX propeptide Antibody

Images

 
Western Blot: LOX propeptide Antibody [NBP1-30012] - Western blot on cytoplasmic (c) and nuclear (n) extracts of NIH 3T3, ras-transformed (RS 485), PMEF and TGM-1 mouse fibroblast cells.
Immunohistochemistry-Paraffin: LOX propeptide Antibody [NBP1-30012] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human skin using LOX propeptide antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). ...read more
Simple Western: LOX propeptide Antibody [NBP1-30012] - Simple Western lane view shows a specific band for LOX propeptide in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

LOX propeptide Antibody Summary

Immunogen
A synthetic peptide made to an internal region of mouse LOX propeptide (within residues 135-155). [Swiss-Prot P28301]
Localization
Secreted extracellular space. Cytoplasm and nucleus (Contente, et al).
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LOX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.35 ug/ml
  • Simple Western 1:200
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
Application Notes
This LOX propeptide antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Western blot, where a band is seen approx. 50 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NIH 3T3 Lysate (NB800-PC8)
Publications
Read Publications using
NBP1-30012 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human and mouse. Immunogen has 100% homology with rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LOX propeptide Antibody

  • lysyl oxidaseEC 1.4.3.13
  • MGC105112
  • protein-lysine 6-oxidase

Background

Lysyl oxidase (LOX) is a critical enzyme in extracellular matrix biosynthesis as well as in the maintainance of normal bone phenotype. It is synthesized/secreted as a 50-kD proenzyme 'pro-LOX' which is then processed to 30-kD mature enzyme 'LOX' and 18-kD lysyl oxidase propeptide 'LOX-PP' by extracellular procollagen C-proteinases (BMP1, Tll1, and Tll2). LOX-PP has been proposed to implicate in the maintenance of inactive state of LOX within the secretory pathway and to act as intramolecular chaperones which facilitate the accurate protein folding/targeting to correct destinations. LOX-PP involves tumor suppression by inhibiting RAS-dependent cellular transformation and its expression has also been associated with inhibition of anchorage-independent progression as well as migration and growth suppression xenografts in Her-2/neu-driven breast cancer. LOX-PP also inhibits PI3K/AKT and ERK1/2 MAP kinase pathways, as well as the levels of downstream NF-kB/cyclin D1 signals in certain cancers - breast, pancreatic, lung, prostate and oral cancers. LOX-PP has also been shown to inhibit DNA synthesis in rat's VSMCs cultures, osteoblast proliferation and FGF-2 signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LOX propeptide Antibody (NBP1-30012)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LOX propeptide Antibody (NBP1-30012) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-30012AF405
Alexa Fluor 488 Labeled NBP1-30012AF488
Alexa Fluor 647 Labeled NBP1-30012AF647
Alexa Fluor 700 Labeled NBP1-30012AF700
Biotin Labeled NBP1-30012B
DyLight 405 Labeled NBP1-30012V
DyLight 488 Labeled NBP1-30012G
DyLight 550 Labeled NBP1-30012R
DyLight 650 Labeled NBP1-30012C
FITC Labeled NBP1-30012F
HRP Labeled NBP1-30012H

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Bioinformatics Tool for LOX propeptide Antibody (NBP1-30012)

Discover related pathways, diseases and genes to LOX propeptide Antibody (NBP1-30012). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for LOX propeptide Antibody (NBP1-30012)

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Blogs on LOX propeptide.

LOX propeptide: A novel peptide cancer therapeutic
Lysyl oxidase, also known as LOX, is a copper-dependent enzyme that cross-links collagen and elastin through the oxidative deamination of peptidyl lysine (collagen and elastin) and hydroxylysine (collagen only) residues, thereby playing a critical rol...  Read full blog post.

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Bioinformatics

Gene Symbol LOX
Entrez
Uniprot