LOX Antibody

Western Blot: LOX Antibody [NB100-2528] - Analysis of LOX in human kidney

Immunocytochemistry/Immunofluorescence: LOX Antibody [NB100-2528] - LOX antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Discontinued

LOX Antibody Summary

• Immunogen: A synthetic peptide made to an internal region of the human LOX protein (within residues 300-350). [Swiss-Prot P28300]
• Localization: Nucleus, cytoplasm, secreted.
• Predicted Species: Rat (100%), Porcine (92%), Bovine (100%), Zebrafish (92%), Xenopus (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LOX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Application Notes: This LOX antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot analysis. In Western blot a band is observed ~50 kDa representing the Lox propeptide. In ICC/IF, cytoplasmic staining was observed in HeLa cells.
• Control:

  MAP7 Antibody
  Human Kidney Whole Tissue Lysate (Adult Whole Normal)
  Mouse Kidney Whole Tissue Lysate (Adult Whole Normal)
  LOX Overexpression Lysate

• Control Peptide:

  LOX Peptide (NB100-2528PEP)

Reactivity Notes

Human and mouse. Predicted to react with rat and bovine based on 100% sequence homology. Immunogen sequence has 92% homology to porcine, Xenopus and Zebrafish.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LOX Antibody

• AAT10
• Lysyl Oxidase/LOX
• lysyl oxidaseEC 1.4.3.13
• MGC105112
• protein-lysine 6-oxidase

Background

LOX (lysyl oxidase) is a copper-dependent amine oxidase enzyme responsible for post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOX is synthesized as an inactive 50 kD proenzyme that is N-glycosylated in ER/Golgi complex and then secreted into extracellular environment, where it is processed by pro-collagen C-proteinases (mammalian Tolloids and BMP-1) into an active enzyme (28-32 kD) and an 18 kD propeptide (LOX-PP) which can be N-glycosylated intracellularly into a ~35 kD form. LOX activity is critical in maintaining the tensile as well as elastic features of the connective tissues of skeletal, pulmonary and cardiovascular systems. LOX deficiency leads to intra-gestational lethality in LOX-/- mice wherein the pups exhibits aortic aneurysms/tortuosity/rupture. LOX expression is highly regulated during normal development; and, its aberrant expression or activity has been documented in a range of diseases predominantly associated with the ECM such as Cutis laxa or OHS (occipital horn syndrome), Menkes' syndrome, myocardial ischaemia/heart failure, atherosclerosis, scleroderma, liver cirrhosis, Pseudoexfoliation syndrome and glaucoma, Alzheimer's/non-Alzheimer's dementia, Wilson's disease, primary biliary cirrhosis as well as in many type of cancers, wherein LOX implicates in promoting tumor cell invasion and metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LOX Antibody (NB100-2528)(1)

Publication using NB100-2528

• Contente S, Yeh TJ, Friedman RM. Tumor suppressive effect of lysyl oxidase proenzyme. Biochim Biophys Acta 2009-07-01 [PMID: 19410608]

Bioinformatics

• Gene Symbol: LOX
• Entrez:
  • 4015(Human)
  • 16948(Mouse)
• Uniprot:
  • P28300(Human)