LOX Antibody

Images

 
Western Blot: LOX Antibody [NB100-2528] - Analysis of LOX in human kidney
Immunocytochemistry/ Immunofluorescence: LOX Antibody [NB100-2528] - LOX antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Xp, ZeSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

LOX Antibody Summary

Immunogen
A synthetic peptide made to an internal region of the human LOX protein (within residues 300-350). [Swiss-Prot P28300]
Localization
Nucleus, cytoplasm, secreted.
Predicted Species
Rat (100%), Porcine (92%), Bovine (100%), Zebrafish (92%), Xenopus (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LOX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
This LOX antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot analysis. In Western blot a band is observed ~50 kDa representing the Lox propeptide. In ICC/IF, cytoplasmic staining was observed in HeLa cells.
Positive Control
MAP7 Antibody (NB100-74618)
DC-SCRIPT/ZNF366 RNAi (H00167465-R01)
Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (NB110-40584)
Kidney Lysate (NB820-59231)
Kidney Lysate (NB820-59661)
LOX Lysate (NBL1-12640)
Control Peptide
LOX Peptide (NB100-2528PEP)
Publications
Read Publication using NB100-2528.

Reactivity Notes

Human and mouse. Predicted to react with rat and bovine based on 100% sequence homology. Immunogen sequence has 92% homology to porcine, Xenopus and Zebrafish.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LOX Antibody

  • lysyl oxidaseEC 1.4.3.13
  • MGC105112
  • protein-lysine 6-oxidase

Background

LOX (lysyl oxidase) is a copper-dependent amine oxidase enzyme responsible for post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOX is synthesized as an inactive 50 kD proenzyme that is N-glycosylated in ER/Golgi complex and then secreted into extracellular environment, where it is processed by pro-collagen C-proteinases (mammalian Tolloids and BMP-1) into an active enzyme (28-32 kD) and an 18 kD propeptide (LOX-PP) which can be N-glycosylated intracellularly into a ~35 kD form. LOX activity is critical in maintaining the tensile as well as elastic features of the connective tissues of skeletal, pulmonary and cardiovascular systems. LOX deficiency leads to intra-gestational lethality in LOX-/- mice wherein the pups exhibits aortic aneurysms/tortuosity/rupture. LOX expression is highly regulated during normal development; and, its aberrant expression or activity has been documented in a range of diseases predominantly associated with the ECM such as Cutis laxa or OHS (occipital horn syndrome), Menkes' syndrome, myocardial ischaemia/heart failure, atherosclerosis, scleroderma, liver cirrhosis, Pseudoexfoliation syndrome and glaucoma, Alzheimer's/non-Alzheimer's dementia, Wilson's disease, primary biliary cirrhosis as well as in many type of cancers, wherein LOX implicates in promoting tumor cell invasion and metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LOX Antibody (NB100-2528)(1)


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LOX Antibody (NB100-2528) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2528AF405
Alexa Fluor 488 Labeled NB100-2528AF488
Alexa Fluor 647 Labeled NB100-2528AF647
Alexa Fluor 700 Labeled NB100-2528AF700
Biotin Labeled NB100-2528B
DyLight 405 Labeled NB100-2528V
DyLight 488 Labeled NB100-2528G
DyLight 550 Labeled NB100-2528R
DyLight 650 Labeled NB100-2528C
FITC Labeled NB100-2528F
HRP Labeled NB100-2528H

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Bioinformatics Tool for LOX Antibody (NB100-2528)

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Research Areas for LOX Antibody (NB100-2528)

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Blogs on LOX.

LOX: A prime enzyme
LOX is a copper-dependent amine oxidase enzyme that executes post-translational oxidative deamination on peptidyl lysine residues in precursors of fibrous collagen and elastin. LOX is secreted into the extracellular environment in an inactive form, wh...  Read full blog post.

Understanding DNA Recombination with Cre-Lox
Cyclization recombination enzyme (Cre) is a member of the extensive family of recombinases and recognizes a 34 bp sequence motif from PI bacteriophage referred to as LoxP. The Cre enzyme works to cleanly excise an intervening DNA fragment that is flan...  Read full blog post.

Cre/Lox: The Genomic Utility Knife
Cre (Cyclization recombination enzyme) is a member of the large family of recombinases. Cre recognizes Lox site loxP, a 34 bp sequence motif from the PI bateriophage. If a DNA segment is flanked by two loxP sites in the same orientation, Cre neatly ex...  Read full blog post.

LOX propeptide: A novel peptide cancer therapeutic
Lysyl oxidase, also known as LOX, is a copper-dependent enzyme that cross-links collagen and elastin through the oxidative deamination of peptidyl lysine (collagen and elastin) and hydroxylysine (collagen only) residues, thereby playing a critical rol...  Read full blog post.

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Bioinformatics

Gene Symbol LOX
Entrez
Uniprot