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MAP7 Antibody

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Western Blot: MAP7 Antibody [NB100-74618] - Detection of Human MAP7 on HeLa whole cell lysate using NB100-74618. MAP7 was successfully immunoprecipitated by rabbit anti-MAP7 antibody NB100-74661.
Independent Antibodies: Western Blot: MAP7 Antibody [NB100-74618] - Detection of Human MAP7 on HeLa whole cell lysate using NB100-74618. MAP7 was successfully immunoprecipitated by rabbit anti-MAP7 antibody ...read more
Immunohistochemistry-Paraffin: MAP7 Antibody [NB100-74618] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-MAP7 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

        

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MAP7 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 720 and 749 of human microtubule-associated protein 7 using the numbering given in entry NP_003971.1 (GeneID 9053).
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP7
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Immunoprecipitation Not recommended.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAP7 Antibody

  • E-MAP-115dJ325F22.2 (microtubule-associated protein 7 (EMAP115, E-MAP-115))
  • ensconsin
  • Epithelial microtubule-associated protein of 115 kDa
  • MAP-7
  • microtubule-associated protein 7EMAP115

Background

The product of this gene is a microtubule-associated protein that is predominantly expressed in cells of epithelial origin. Microtubule-associated proteins are thought to be involved in microtubule dynamics, which is essential for cell polarization and differentiation. This protein has been shown to be able to stabilize microtubules, and may serve to modulate microtubule functions. Studies of the related mouse protein also suggested an essential role in microtubule function required for spermatogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: SGSM3 Antibody [NBP2-13304] - Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.
Western Blot: SGSM3 Antibody [NBP2-13304] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Kinesin 5B Antibody
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Western Blot: Kinesin 5B Antibody [NBP2-21667] - Glucocorticoid promoted microtubule destabilization and following transport impairment. The hippocampus of mice exposed to rapamycin (8 mg/kg) for 2 days before corticosterone (10 mg/kg) for 24 h was collected and lysed. alpha-tubulin was co-immunoprecipitated with an anti-kinesin-1 antibody (the left side). Expression of kinesin-1 and alpha-tubulin in total cell lysates is shown in the right side. ** indicates p < 0.01 vs. vehicle. # indicates p < 0.05 vs. corticosterone alone. n = 4. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41419-018-1172-y) licensed under a CC-BY licence.
Immunohistochemistry: Kinesin 5B Antibody [NBP2-21667] - 40X in human brain at pH 6. Tissue: Human Brain.
Western Blot: Kinesin 5B Antibody [NBP2-21667] - Western Blot of Kinesin 5B antibody. Lane 1: 500 ng of truncated kinesin 1 protein. Lane 2: 20 ug of Mouse Brain lysate. Lane 3: 10 ug of Mouse Brain lysate. Primary antibody: kinesin1 antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:40,000 for 1hr at RT.Block: 5% BLOTTO 1 hr at RT.Predicted/Observed size: 72 kDa, ~70 kDa for Kinesin 5B. Other band(s): degradation.
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Immunohistochemistry-Paraffin: RPS14 Antibody [NBP1-84847] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: RPS14 Antibody [NBP1-84847] - Staining of human colon shows moderate to strong cytoplasmic positivity in glandular cells.
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
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NBP2-37607
Immunohistochemistry-Paraffin: MTHFR Antibody (5D3) [NBP2-37607] - Analysis of intima cancer tissues (left) and prostate tissues (right) using MTHFR mouse mAb with DAB staining.
ELISA: MTHFR Antibody (5D3) [NBP2-37607] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Western Blot: MTHFR Antibody (5D3) [NBP2-37607] - Western blot analysis using MTHFR mAb against HEK293 (1) and MTHFR(AA: 339-499)-hIgGFc transfected HEK293 (2) cell lysate.
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, WB
PD-ECGF/Thymidine
Phosphorylase Antibod ...
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Immunohistochemistry-Paraffin: PD-ECGF/Thymidine Phosphorylase Antibody (PGF 44C) [NB100-2737] - Analysis of Thymidine Phosphorylase formalin-fixed paraffin embedded human breast carcinoma.
Immunohistochemistry: PD-ECGF/Thymidine Phosphorylase Antibody (PGF 44C) [NB100-2737] - Immunohistochemical staining of thymidine phosphorylase (TP) expression and related markers in benign lymph node. In benign lymph node, TP expression highlights the meshwork of macrophages (arrow in inset; 400x) and endothelial cells (arrowhead in inset; 400x). Images are from an Olympus BX41 microscope (Olympus Corp., Tokyo, Japan) processed with Qcapture Pro 5.1 (QImaging, Surrey, BC, Canada). Image collected and cropped by CiteAb from the following publication (http://www.hindawi.com/journals/ah/2011/875135/) licensed under a CC-BY licence.
Immunohistochemistry: PD-ECGF/Thymidine Phosphorylase Antibody (PGF 44C) [NB100-2737] - Immunohistochemical staining of thymidine phosphorylase (TP) expression and related markers in benign lymph node in lymph node with malignant mycosis fungoides/Sezary syndrome cells (LN-MF). LN-MF: hematoxylin and eosin (H&E) stain. Images are from an Olympus BX41 microscope (Olympus Corp., Tokyo, Japan) processed with Qcapture Pro 5.1 (QImaging, Surrey, BC, Canada). Image collected and cropped by CiteAb from the following publication (http://www.hindawi.com/journals/ah/2011/875135/) licensed under a CC-BY licence.
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
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(IU2H10) - BSA Free
NB400-156
Dual RNAscope ISH-IHC: MRP1 Antibody (IU2H10) [NB400-156] - Formalin-fixed paraffin-embedded tissue sections of human testis were probed for MRP1 mRNA (ACD RNAScope Probe, catalog # 435591; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse monoclonal (Novus Biologicals catalog # NB400-156) at 7ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to Leydig cells and seminiferous tubule cells.
Western Blot: MRP1 Antibody (IU2H10) [NB400-156] - Vector-transfected HEK293 cells 2: MRP1-transfected cells, prep1 3: MRP1-transfected cells, prep2 (the lower band is a degradation product.)
Immunocytochemistry/Immunofluorescence: MRP1 Antibody (IU2H10) [NB400-156] - The MRP-1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.
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Species: Hu, Mu, Rt
Applications: CyTOF-ready, Dual ISH-IHC, ELISA, Flow, ICC/IF, IHC, IP, WB
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H00001806-M01
ELISA: DPYD Antibody (7D4) [H00001806-M01] - Detection limit for recombinant GST tagged DPYD is approximately 0.1ng/ml as a capture antibody.
Western Blot: DPYD Antibody (7D4) [H00001806-M01] - DPYD monoclonal antibody (M01), clone 7D4. Analysis of DPYD expression in Hela S3 NE.
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(1 Publication)
Species: Hu
Applications: DB, ELISA, ICC/IF, WB
Thymidylate
Synthase Antibody (TY ...
NBP3-08882
Protein Array: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-08882] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Thymidylate Synthase Monospecific Mouse Monoclonal Antibody (TYMS/1884).
Flow Cytometry: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-08882] - Flow Cytometric Analysis of PFA-fixed MOLT4 cells. Thymidylate Synthase Monospecific Mouse Monoclonal Antibody followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).
Flow Cytometry: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-08882] - Flow Cytometric Analysis of PFA-fixed Ramos cells. Thymidylate Synthase Monospecific Mouse Monoclonal Antibody followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PA, WB
ErbB2/Her2
[Unconjugated]
1129-ER
1 μg/lane of Recombinant Human ErbB2 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 125-130 kDa and  220-250 kDa, respectively.
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Applications: BA
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Antibody
AF5510
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H00342184-M07
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Immunocytochemistry/Immunofluorescence: FMN1 Antibody (4F4) [H00342184-M07] - Analysis of monoclonal antibody to FMN1 on HeLa cell. Antibody concentration 10 ug/ml
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Species: Hu
Applications: ELISA, ICC/IF, WB
PYK2/FAK2 [p
Tyr402] Antibody (592 ...
MAB6210
Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line untreated (-) or treated (+) with 0.2 mg/mL Pervanadate (PV) for 30 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-PYK2/FAK2 (Y402) at approximately 113 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Phospho-PYK2/FAK2 (Y402) Monoclonal Antibody (Catalog # MAB6210). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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(1 Review)
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Applications: Simple Western, WB
Caldesmon/CALD1
Antibody (h-CALD)
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Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421] - Analysis of Human uterus tissue using Caldesmon/CALD1 antibody (h-CALD). Image from verified customer review.
Western Blot: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421] - Western Blot Analysis of human Ovary tissue using Caldesmon/CALD1 antibody (h-CALD).
Simple Western: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421] - Simple Western lane view shows a specific band for Caldesmon/CALD1 in 0.2 mg/ml of h. Aorta lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
(1 Review)
Species: Hu
Applications: Flow, ICC/IF, IF, IHC, IHC-P, Simple Western, WB
MDR1/ABCB1
Antibody (SN06-42)
NBP2-67667
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of paraffin-embedded human liver tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.
Immunohistochemistry-Frozen: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Mouse (B6) liver tissue. Fixed in ice-cold methanol for 10 minutes. Blocking in 10% serum and 1% BSA. Primary antibody at 5 ug/mL, overnight incubation. Secondary antibody at 2 ug/mL for 1.5 hour (Invitrogen A-11012). Mounting with Vector Vectashield with DAPI. Image from a verified customer review.
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of paraffin-embedded mouse kidney tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
TOR/mTOR [p
Ser2448] Antibody
NB600-607
Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Brown arrow= IBa1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and IBA1 positive cells. IHC-P Image submitted by a verified customer review.
Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Rapamycin reduces mTOR phosphorylation induced by TII. Whole-cell lysates were prepared from astrocytes activated with TII for 2 h. 10 nM Rapamycin was added at the beginning of the experiment and equal amounts of protein were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR) (upper gel) and consequently for beta-actin (lower gel). Image collected and cropped by CiteAb from the following publication (http://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-1), licensed under a CC-BY licence.
N/A: TOR/mTOR [p Ser2448] Antibody [NB600-607]
(2 Reviews)
(9 Publications)
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, NULL, WB
MDMX Antibody
NB100-556
Western Blot: MDMX Antibody [NB100-556] - Detection of human HdmX/MDM4 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HdmX/MDM4 antibody NB100-556 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Immunoprecipitation: MDMX Antibody [NB100-556] - Detection of human HdmX/MDM4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HdmX/MDM4 antibody NB100-556 (lot NB100-556-4) used for IP at 6 ug per reaction. HdmX/MDM4 was also immunoprecipitated by a previous lot of this antibody (lot NB100-556-3) and another rabbit anti-HdmX/MDM4 antibody. For blotting immunoprecipitated HdmX/MDM4, NB100-556 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: MDMX Antibody [NB100-556] - Analysis using the Biotin conjugate of NB100-556. Detection of Jurkat (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 30 seconds.
(32 Publications)
Species: Hu
Applications: IP, WB
Lipoprotein
Lipase/LPL Antibody [ ...
AF7197
Lipoprotein Lipase/LPL was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human/Mouse Lipoprotein Lipase/LPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7197) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line and NMuMG mouse mammary gland epithelial cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Lipoprotein Lipase/LPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lipoprotein Lipase/LPL at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Lipoprotein Lipase/LPL was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Goat Anti-Human Lipoprotein Lipase/LPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7197) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. This application has not been tested in mouse samples.
(2 Publications)
Species: Hu, Mu
Applications: ICC, IHC, WB
TBC1D9 Antibody
NBP2-13415
Immunohistochemistry-Paraffin: TBC1D9 Antibody [NBP2-13415] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunocytochemistry/Immunofluorescence: TBC1D9 Antibody [NBP2-13415] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Species: Hu
Applications: ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol MAP7
Entrez
Uniprot