LNK/SH2B3 Antibody

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, SH-SY5Y human neuroblastoma cell line, Ramos human Burkitt's lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human LNK/SH2B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5888) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LNK/SH2B3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

LNK/SH2B3 Antibody Summary

• Immunogen: E. coli-derived recombinant human LNK/SH2B3
  Gln427-Leu575
  Accession # Q9UQQ2
• Specificity: Detects human LNK/SH2B3 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SH2B3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LNK/SH2B3 Antibody

• IDDM20
• LNK
• LNKSH2B adapter protein 3
• Lymphocyte adapter protein
• Lymphocyte-specific adapter protein Lnk
• SH2B adaptor protein 3
• SH2B3
• Signal transduction protein Lnk

Background

LNK (also SH2B adaptor protein 3) is a protein intermediary, or LiNK, between cell surface molecules (TPO R; EPO R; SCFR; TCR) and downstream signaling molecules such as PI3 K, Grb2 and Jak2. Although its predicted MW is 63 kDa, it runs anomalously at 75 kDa in SDS‑PAGE. LNK is found in hematopoietic cells, and serves to limit responsiveness to growth factor stimulation. Human LNK is 575 amino acids (aa) in length. It contains an N-terminal Phe-zipper segment that likely serves as a dimerization domain (aa 24‑81), a PH domain that may interact with membrane lipids (aa 197‑305), and an SH2 domain that binds phosphoTyr (aa 364‑462).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LNK/SH2B3 Antibody (AF5888)(3)

Publications using AF5888

• LW Ding, QY Sun, JJ Edwards, LT Fernández, XB Ran, SQ Zhou, RA Scolyer, JS Wilmott, JF Thompson, N Doan, JW Said, N Venkatacha, JF Xiao, XY Loh, M Pein, L Xu, DW Mullins, H Yang, DC Lin, HP Koeffler LNK suppresses interferon signaling in melanoma Nat Commun, 2019-05-20;10(1):2230. 2019-05-20 [PMID: 31110180] (IHC, Western Blot, Human)
• Ying Cheng, Kudakwashe Chikwava, Chao Wu, Haibing Zhang, Anchit Bhagat, Dehua Pei et al. LNK/SH2B3 regulates IL-7 receptor signaling in normal and malignant B-progenitors Journal of Clinical Investigation 4/1/2016 [PMID: 26974155]
• Ding , L-W, Sun , Q-Y, Lin , D-C, Chien , W, Hattori , N, Dong , X-M, Gery , S, Garg , M, Doan , N B, Said , J W, Xiao , J-F, Yang , H, Liu , L-Z, Meng , X, Huang , R Y-J, Tang , K, Koeffler , H P LNK (SH2B3): paradoxical effects in ovarian cancer. Oncogene, 2014-04-07;34(11):1463-74. 2014-04-07 [PMID: 24704825] (IHC, Human)

Bioinformatics

• Gene Symbol: SH2B3
• Uniprot:
  • Q9UQQ2(Human)