LNK/SH2B3 Antibody [Alexa Fluor® 350]

• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

LNK/SH2B3 Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human LNK/SH2B3
  Gln427-Leu575
  Accession # Q9UQQ2
• Specificity: Detects human LNK/SH2B3 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

LNK (also SH2B adaptor protein 3) is a protein intermediary, or LiNK, between cell surface molecules (TPO R; EPO R; SCFR; TCR) and downstream signaling molecules such as PI3 K, Grb2 and Jak2. Although its predicted MW is 63 kDa, it runs anomalously at 75 kDa in SDS‑PAGE. LNK is found in hematopoietic cells, and serves to limit responsiveness to growth factor stimulation. Human LNK is 575 amino acids (aa) in length. It contains an N-terminal Phe-zipper segment that likely serves as a dimerization domain (aa 24‑81), a PH domain that may interact with membrane lipids (aa 197‑305), and an SH2 domain that binds phosphoTyr (aa 364‑462).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.