LNK/SH2B3 Antibody (OTI2D8) - Azide and BSA Free

Western Blot: LNK/SH2B3 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72514] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LNK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LNK.

Immunocytochemistry/Immunofluorescence: LNK/SH2B3 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72514] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LNK.

Immunohistochemistry: LNK/SH2B3 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72514] - Staining of paraffin-embedded Human Kidney tissue using anti-LNK mouse monoclonal antibody.

Flow Cytometry: LNK/SH2B3 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72514] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-LNK antibody, and then analyzed by flow cytometry.

Immunohistochemistry: LNK/SH2B3 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72514] - Staining of paraffin-embedded Human pancreas tissue using anti-LNK mouse monoclonal antibody.

Flow Cytometry: LNK/SH2B3 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72514] - Analysis of Hela cells, using anti-LNK antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI2D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

LNK/SH2B3 Antibody (OTI2D8) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human SH2B3 (NP_005466) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SH2B3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for LNK/SH2B3 Antibody (OTI2D8) - Azide and BSA Free

• IDDM20
• LNK
• LNKSH2B adapter protein 3
• Lymphocyte adapter protein
• Lymphocyte-specific adapter protein Lnk
• SH2B adaptor protein 3
• SH2B3
• Signal transduction protein Lnk

Background

T-cell activation requires stimulation of the T-cell receptor (TCR; see MIM 186880)-CD3 (see CD3Z; MIM 186780) complex, followed by recruitment of an array of intracellular signaling proteins (e.g., GRB2 (MIM 108355) and PLCG1 (MIM 172420)). Mediating the interaction between the extracellular receptors and intracellular signaling pathways are adaptor proteins such as LAT (MIM 602354), TRIM (MIM 604962), and LNK.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SH2B3