LMO2 Antibody [Unconjugated]

Images

 
LMO2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726) at 10 µg/mL for 3 hours at room temperature. ...read more
K562 chronic myelogenous leukemia human cell line was stained with Goat Anti-Human LMO2 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2726, filled histogram) or control antibody (Catalog # AB-108-C, open ...read more
Human PBMC were stained with Goat Anti-Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

LMO2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human LMO2
Ser2-Ile158
Accession # P25791
Specificity
Detects human LMO2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LMO2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2726 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LMO2 Antibody [Unconjugated]

  • Cysteine-rich protein TTG-2
  • LIM domain only 2 (rhombotin-like 1)
  • LIM domain only protein 2
  • LMO2
  • LMO-2
  • RBTN2
  • RBTN2rhombotin-2
  • RBTNL1
  • RBTNL1rhombotin-like 1
  • RHOM2
  • RHOM2T-cell translocation protein 2
  • T-cell translocation gene 2
  • TTG2
  • TTG2rhombotin 2

Background

LMO2, also known as Rhombotin-2 and T-cell translocation protein-2, is a transcriptional co‑factor that is required for hematopoietic and endothelial development. It contains two LIM domains that are characterized by a zinc binding, cysteine rich motif consisting of two tandemly repeated zinc fingers. LMO2 does not interact directly with DNA but is involved in the assembly of multiprotein transcription factor complexes. Human and mouse LMO2 share 99% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-01488
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-47492
Species: Pm, Hu
Applications: ChIP, ELISA, ICC/IF, IHC, IHC-P, WB
NBP2-48693
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-47940
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC/IF, IF, IHC, IHC-P, Simple Western, WB
NBP1-31362
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, KO, WB
H00004004-M01
Species: Hu
Applications: ELISA, ICC/IF, WB
H00009079-M02
Species: Hu
Applications: ELISA, ICC/IF, WB
MAB7650
Species: Mu
Applications: IHC, WB
AF2046
Species: Hu, Mu
Applications: ICC, IHC, Simple Western, WB
AF6116
Species: Hu
Applications: ICC, WB
NBP1-84843
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-27163
Species: Bv, Ca, Eq, Hu, Mu, Pm
Applications: Flow, WB
NB100-56424
Species: Hu, Mu, Rt
Applications: WB
AF2046
Species: Hu, Mu
Applications: ICC, IHC, Simple Western, WB
AF3889
Species: Hu, Mu
Applications: WB
NBP1-89105
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-80833
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
H00009260-M01
Species: Hu, Rt
Applications: ELISA, ICC/IF, IP, KD, S-ELISA, WB
NBP1-06983
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, PEP-ELISA, WB
AF2726
Species: Hu
Applications: WB, Flow, ICC/IF

Publications for LMO2 Antibody (AF2726)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ChIP, ChIP-Seq, Other, Western Blot.


Filter By Application
ChIP
(1)
ChIP-Seq
(1)
Other
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(3)
Mouse
(3)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF2726 Applications Species
KI Hirano, H Hosokawa, M Koizumi, Y Endo, T Yahata, K Ando, K Hozumi LMO2 is essential to maintain the ability of progenitors to differentiate into T-cell lineage in mice Elife, 2021;10(0):. 2021 [PMID: 34382935] (ChIP, Mouse) ChIP Mouse
N Bery, CJR Bataille, A Russell, A Hayes, F Raynaud, S Milhas, S Anand, H Tulmin, A Miller, TH Rabbitts A cell-based screening method using an intracellular antibody for discovering small molecules targeting the translocation protein LMO2 Science Advances, 2021;7(15):. 2021 [PMID: 33837087]
M Nafria, P Keane, ES Ng, EG Stanley, AG Elefanty, C Bonifer Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells Cell Rep, 2020;31(8):107691. 2020 [PMID: 32460028] (ChIP-Seq, Human) ChIP-Seq Human
VS Stanulovic, P Cauchy, SA Assi, M Hoogenkamp LMO2 is required for TAL1 DNA binding activity and initiation of definitive haematopoiesis at the haemangioblast stage Nucleic Acids Res., 2017;45(17):9874-9888. 2017 [PMID: 28973433] (Western Blot, Mouse) Western Blot Mouse
Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors Nat Commun, 2016;7(0):13396. 2016 [PMID: 27869129] (Other, Human) Other Human
Treanor L, Zhou S, Janke L, Churchman M, Ma Z, Lu T, Chen S, Mullighan C, Sorrentino B Interleukin-7 receptor mutants initiate early T cell precursor leukemia in murine thymocyte progenitors with multipotent potential. J Exp Med, 2014;211(4):701-13. 2014 [PMID: 24687960] (Western Blot, Mouse) Western Blot Mouse
Hirose K, Inukai T, Kikuchi J, Furukawa Y, Ikawa T, Kawamoto H, Oram SH, Gottgens B, Kiyokawa N, Miyagawa Y, Okita H, Akahane K, Zhang X, Kuroda I, Honna H, Kagami K, Goi K, Kurosawa H, Look AT, Matsui H, Inaba T, Sugita K Aberrant induction of LMO2 by the E2A-HLF chimeric transcription factor and its implication in leukemogenesis of B-precursor ALL with t(17;19). Blood, 2010;116(6):962-70. 2010 [PMID: 20519628] (Western Blot, Human) Western Blot Human

Reviews for LMO2 Antibody (AF2726) (0)

There are no reviews for LMO2 Antibody (AF2726). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LMO2 Antibody (AF2726) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional LMO2 Products

Bioinformatics Tool for LMO2 Antibody (AF2726)

Discover related pathways, diseases and genes to LMO2 Antibody (AF2726). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for LMO2 Antibody (AF2726)

Discover more about diseases related to LMO2 Antibody (AF2726).
 

Pathways for LMO2 Antibody (AF2726)

View related products by pathway.

PTMs for LMO2 Antibody (AF2726)

Learn more about PTMs related to LMO2 Antibody (AF2726).

Blogs on LMO2

There are no specific blogs for LMO2, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our LMO2 Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol LMO2
Uniprot